Alexa Fluor® 647 Anti-alpha Tubulin 抗体 [EP1332Y] - Microtubule Marker
Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker
- RabMAb
- Recombinant
- 詳細を見る
5
(3 Reviews)
|
(13 Publications)
Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker - Alexa Fluor® 647 conjugated (ab190573) is a rabbit recombinant monoclonal antibody detecting alpha Tubulin in ICC/IF. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
別名を表示する
TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain
- ICC
Lab
Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
ab197235 staining FOXA1 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197235 at a 1/100 dilution (shown in green) and ab190573, Rabbit monoclonal to alpha-Tubulin (Alexa Fluor® 647), at a 1/250 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat IDH2 knockout cells labeling IDH2 with ab212122 (green) at 1 μg/ml. Cells were counterstained with ab190573 Anti-Tubulin (Alexa Fluor® 647) at 1 : 250 (2 μg/ml) (magenta). The nuclear counterstain was DAPI (blue).
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
ab190573 staining alpha Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab190573 at 1/500 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
ab190573 staining alpha Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190573 at a working dilution of 1 in 100 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
This product also gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
ab1220 staining Histone H3 (di methyl K9) in HeLa (Human cervix adenocarcinoma epithelial cell) cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab1220 at 5 µg/ml and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2 µg/ml (shown in red). The secondary antibody (shown in green) was ab150117, Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. This product also gave a positive outcome under the same testing conditions in HeLa cells fixed with 100% methanol (5 mins).
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
Immunofluorescent analysis of 4% PFA-fixed 0.1% Triton X-100 permeabilized A549 WT and A549-SLC2A1 KO cells labelling Glucose Transporter GLUT1 with ab209449 at 1 μg/ml concentration (Green). Cells were counterstained with ab190573 Anti-Tubulin (Alexa Fluor® 647) at 1 : 250 (2 μg/ml) (Magenta). The nuclear counterstain was DAPI (Blue).
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
Immunofluorescent analysis of 4% PFA-fixed 0.1% Triton X-100 permeabilized HEK-293 WT and HEK-293-HK1 KO cells labelling Hexokinase 1 with ab184818 at 0.2 μg/ml concentration (Green). Cells were counterstained with ab190573 Anti-Tubulin (Alexa Fluor® 647) at 1 : 250 (2 μg/ml) (Magenta). The nuclear counterstain was DAPI (Blue).
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
ab314401 staining PBR in Hela cells, with negative expression in Hela-TSPO KO cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab314401 at 0.2 μg/ml (shown in green). ab190573, rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647) antibody (shown in red) was used at 5 μg/ml for structural counterstaining. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. This product also gave a positive signal in 100% methanol (5 min) fixed HeLa cells and no signal Hela-TSPO KO under the same testing conditions.
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
Immunofluorescent analysis of 100% methanol-fixed 0.1% Triton X-100 permeabilized Hap1 WT and Hap1-PRDX2 KO cells labelling Peroxiredoxin 2 with ab197536 (green) at 1 μg/ml. Cells were counterstained with ab190573 Anti-Tubulin (Alexa Fluor® 647) at 1 : 250 (2 μg/ml) (magenta). The nuclear counterstain was DAPI (Blue).
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
ab281778 staining LAMP1 in HAP1 cells, with negative expression in LAMP1 knockout HAP1 cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab281778 at 0.2 μg/ml (shown in green). ab190573, rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647) (shown in magenta) was used at 5 μg/ml for structural counterstaining.
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
- ICC
Lab
Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
ab202295 staining alpha smooth muscle Actin (ACTA2) in wild-type NIH/3T3 cells (top panel) and ACTA2 knockout NIH/3T3 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab202295 at at 5µg/ml (shown in green) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor® 647), pre-adsorbed at 1/250 dilution (shown in magenta) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
- ICC
Lab
Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
ab202509 staining alpha smooth muscle Actin (ACTA2) in wild-type NIH/3T3 cells (top panel) and ACTA2 knockout NIH/3T3 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab202509 at at 5µg/ml (shown in magenta) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor® 647), pre-adsorbed at 1/250 dilution (shown in green) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
- ICC
Lab
Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
ab202295 staining staining alpha smooth muscle Actin (ACTA2) in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab202295 at 5µg/ml (shown in green) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor® 647), pre-adsorbed at 1/250 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
- ICC
Lab
Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
ab277270 staining pan Cytokeratin cells. The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab277270 at at 1µg/ml (shown in green) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor® 647), pre-adsorbed at 1/250 dilution (shown in magenta) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 minutes).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)
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578 PE
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker
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565 Alexa Fluor® 555
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Anti-alpha Tubulin antibody [EP1332Y] - BSA and Azide free
Reactivity data
製品の詳細
What is this antibody validated in?
Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab190573) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
Trusted by the scientific community
Anti-alpha Tubulin [EP1332Y] - Microtubule Marker Alexa Fluor® 647 (ab190573) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.
Related products
Antibody clone EP1332Y is also available pre-conjugated to a variety of labels for your convenience – Anti-alpha Tubulin Alexa Fluor® 647 [EP1332Y] - Microtubule Marker (ab190573).
Other related products
We have a range of other formats of antibody clone [EP1332Y] also available for your convenience: ab52866, Alexa Fluor® 488 - ab185031, Alexa Fluor® 647 - ab190573, Alexa Fluor® 594 - ab202272, PE - ab208752, Carrier free - ab216650, Alexa Fluor® 555 - ab275113
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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精製方法
バッファー組成
出荷温度
短期保存期間
短期保存温度
長期保存温度
分注に関する情報
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補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.
Pathways
Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.
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