Alexa Fluor® 647 Anti-AKT1 + AKT2 + AKT3 抗体 [EPR16798] (ab302553)
![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (AB302553)](https://www.abcam.co.jp/ps/products/302/ab302553/Images/AB302553-488742-anti-akt1--akt2--akt3-antibody-epr16798-intracellular-flow-cytometry-a549.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (AB302553)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR16798] to AKT1 + AKT2 + AKT3
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 647 Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798]
AKT1 + AKT2 + AKT3 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 647 Rabbit monoclonal [EPR16798] to AKT1 + AKT2 + AKT3 -
由来種
Rabbit -
標識
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
アプリケーション
適用あり: Flow Cyt (Intra)more details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow cyt. (Intra): A549 (human lung carcinoma epithelial cell),
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特記事項
ab302553 does not react in immunocytochemistry and immunohistochemistry with human and human, mouse, rat species, respectively.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16798 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 488 Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200619)
- HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854)
- PE Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab210454)
- Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] - Low endotoxin, Azide free (ab214167)
- Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] - BSA and Azide free (ab271930)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302553の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/500. |
ターゲット情報
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機能
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. -
組織特異性
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
ドメイン
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. -
翻訳後修飾
Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
細胞内局在
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. - Information by UniProt
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参照データベース
- Entrez Gene: 10000 Human
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Omim: 164730 Human
- Omim: 164731 Human
- Omim: 611223 Human
- SwissProt: P31749 Human
- SwissProt: P31751 Human
see all -
別名
- AKT antibody
- AKT1 antibody
- AKT1 kinase antibody
see all
画像
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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (AB302553)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed and 90% methanol permeabilized A549 (human lung carcinoma epithelial cell) cells labeling AKT1 + AKT2 + AKT3 with AB302553 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal isotype control - Alexa Fluor® 647 (Black) isotype control, and unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
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![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (AB302553)](https://www.abcam.co.jp/ps/products/302/ab302553/Images/AB302553-488743-anti-akt1--akt2--akt3-antibody-epr16798-intracellular-flow-cytometry-mcf7.jpg)
Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (AB302553)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed and 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labeling AKT1 + AKT2 + AKT3 with AB302553 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal isotype control - Alexa Fluor® 647 (Black) isotype control, and unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
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![Alexa Fluor® 647 Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (AB302553)](https://www.abcam.co.jp/ps/products/302/ab302553/Images/AB302553-1-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 647 Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (AB302553)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab302553 は論文での使用が確認できていません。