Alexa Fluor® 594 Anti-SOX2 抗体 [EPR3131] (ab302657)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SOX2 antibody [EPR3131] (AB302657)](https://www.abcam.co.jp/ps/products/302/ab302657/Images/AB302657-490092-anti-sox2-antibody-epr3131-immunocytochemstry-immunofluorescence-f9-nih-3t3.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SOX2 antibody [EPR3131] (AB302657)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 594 Rabbit monoclonal [EPR3131] to SOX2
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 594 Anti-SOX2 antibody [EPR3131]
SOX2 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 594 Rabbit monoclonal [EPR3131] to SOX2 -
由来種
Rabbit -
標識
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC/IF: F9 (mouse embryonal carcinoma epithelial cell)
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3131 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302657の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/50.
|
| 特記事項 |
|---|
|
ICC/IF
1/50. |
ターゲット情報
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機能
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. -
関連疾患
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. -
配列類似性
Contains 1 HMG box DNA-binding domain. -
翻訳後修飾
Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 20674 Mouse
- SwissProt: P48432 Mouse
- Unigene: 65396 Mouse
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別名
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SOX2 antibody [EPR3131] (AB302657)
Immunofluorescent analysis of 4% paraformaldehyde fixed and 0.1% TritonX-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell）labeling SOX2 with ab302657 at 1/50 dilution (10 μg/mL). Counterstained for tubulin using ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) at 1:200 (2.5 μg/mL). DAPI was used as a counterstain. Confocal image showing nuclear staining in F9 cells.
-ve control: NIH/3T3 (PMID:30762521).
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![Alexa Fluor® 594 Anti-SOX2 antibody [EPR3131] (AB302657)](https://www.abcam.co.jp/ps/products/302/ab302657/Images/AB302657-1-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 594 Anti-SOX2 antibody [EPR3131] (AB302657)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab302657 は論文での使用が確認できていません。