Alexa Fluor® 594 Anti-SDHA 抗体 [2E3GC12FB2AE2] (ab170172)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)](https://www.abcam.co.jp/ps/products/170/ab170172/Images/ab170172-261981-anti-sdha-antibody-2e3gc12fb2ae2-alexa-fluor-594-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)")
Key features and details
- Alexa Fluor® 594 Mouse monoclonal [2E3GC12FB2AE2] to SDHA
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2]
SDHA 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 594 Mouse monoclonal [2E3GC12FB2AE2] to SDHA -
由来種
Mouse -
標識
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog
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免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC/IF: HeLa and HDFn cells.
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特記事項
ab170172 was previously used as a component in the MitoBiogenesis™ ICC Kit. The protocol for this kit is available here.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
2E3GC12FB2AE2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab170172の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/1000.
ab178000 - Mouse monoclonal IgG1 (Alexa Fluor® 594), is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
ICC/IF
1/1000. ab178000 - Mouse monoclonal IgG1 (Alexa Fluor® 594), is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). -
パスウェイ
Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1. -
関連疾患
Defects in SDHA are a cause of mitochondrial complex II deficiency (MT-C2D) [MIM:252011]. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations. Clinical features include psychomotor regression in infants, poor growth with lack of speech development, severe spastic quadriplegia, dystonia, progressive leukoencephalopathy, muscle weakness, exercise intolerance, cardiomyopathy. Some patients manifest Leigh syndrome or Kearns-Sayre syndrome.
Defects in SDHA are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
Defects in SDHA are the cause of cardiomyopathy dilated type 1GG (CMD1GG) [MIM:613642]. CMD1GG is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
配列類似性
Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 281480 Cow
- Entrez Gene: 478634 Dog
- Entrez Gene: 6389 Human
- Entrez Gene: 66945 Mouse
- Entrez Gene: 157074 Rat
- Omim: 600857 Human
- SwissProt: P31039 Cow
- SwissProt: P31040 Human
see all -
別名
- CMD1GG antibody
- DHSA_HUMAN antibody
- Flavoprotein subunit of complex II antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)
ab170172 staining SDHA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab170172 at a 1/1000 dilution (shown in pseudo color red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)](https://www.abcam.co.jp/ps/products/170/ab170172/Images/ab170172-169681-anti-sdha-antibody-2e3gc12fb2ae2-alexa-fluor-594-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)Immunocytochemistry with HDFn (100x) cells.
A) HDFn stained with anti-SDHA Alexa-594 antibody (1.0 µg/mL). B) HDFn stained with Anti-mitochondrial chaperone HSP60 (1/1000, ab46798), Secondary antibody used was goat anti-rabbit Alexa® 488 (1/1000, ab150077). C) DAPI as nuclear stain (1/10000). D) Merge of color channels to show specificity of signal to mitochondria. -
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)](https://www.abcam.co.jp/ps/products/170/ab170172/Images/ab170172-169680-anti-sdha-antibody-2e3gc12fb2ae2-alexa-fluor-594-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)Immunocytochemistry with HeLa cells (100x).
A) HeLa stained with anti-SDHA Alexa-594 antibody (1.0 µg/mL). B) HeLa stained with Anti-HSP60 (1/1000, ab46798), Secondary antibody used was goat anti-rabbit Alexa® 488 (1/1000, ab150077). C) DAPI as nuclear stain (1/10000). D) Merge of color channels to show specificity of signal to mitochondria.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab170172 は論文での使用が確認できていません。