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リコンビナントRabMAb

Alexa Fluor® 594 Anti-PBR 抗体 [EPR5384] (ab303575)

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Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)
  • Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)
  • Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (AB303575)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [EPR5384] to PBR
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 647 Carrier Free PE Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 594 Anti-PBR antibody [EPR5384]
    PBR 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 594 Rabbit monoclonal [EPR5384] to PBR

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

  • アプリケーション

    適用あり: IHC-Fr, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-Fr: Human normal kidney tissue. IHC-P: Human normal colon tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab303575の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
1/100.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-Fr
1/100.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells.

  • 組織特異性

    Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.

  • 配列類似性

    Belongs to the TspO/BZRP family.

  • 細胞内局在

    Mitochondrion membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Benzodiazapine receptor (peripheral) antibody
      • Benzodiazepine peripheral binding site antibody
      • BPBS antibody
      • BZRP antibody
      • DBI antibody
      • IBP antibody
      • Isoquinoline carboxamide-binding protein antibody
      • MBR antibody
      • mDRC antibody
      • Mitochondrial benzodiazepine receptor antibody
      • PBR antibody
      • PBS antibody
      • Peripheral benzodiazepine receptor antibody
      • Peripheral benzodiazepine receptor-related protein antibody
      • Peripheral type benzodiazepine receptor antibody
      • Peripheral-type benzodiazepine receptor antibody
      • pk18 antibody
      • PKBS antibody
      • PTBR antibody
      • Ptbzr antibody
      • PTBZR02 antibody
      • RATPTBZR02 antibody
      • translocator protein (18kDa) antibody
      • Translocator protein antibody
      • Tspo antibody
      • Tspo1 antibody
      • TSPOA_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)

      Immunofluorescence staining of PBR in a section of frozen human normal kidney.

      The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND. The section was incubated at room temperature for 1 hour with AB303575 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)

      Negative immunofluorescence staining of PBR in a section of frozen human normal hippocampus*.

      The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND. The section was incubated at room temperature for 1 hour with AB303575 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)

      Immunofluorescence staining of PBR in a section of formalin-fixed paraffin-embedded human normal colon*.

      Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with AB303575 at 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)

      Negative immunofluorescence staining of PBR in a section of formalin-fixed paraffin-embedded human normal cerebellum.

      Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with AB303575 at 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)
      Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)
    • Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (AB303575)
      Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (AB303575)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab303575 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab303575 は論文での使用が確認できていません。

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