JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB303718

Alexa Fluor® 594 Anti-NCAM1 抗体 [EP2567Y]

Alexa Fluor® 594 Anti-NCAM1 antibody [EP2567Y]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal NCAM1 antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

別名を表示する

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

2 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-NCAM1 antibody [EP2567Y] (AB303718)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-NCAM1 antibody [EP2567Y] (AB303718)

Flow cytometric (Intracellular) analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labeling NCAM1 with ab303718 at 1/500 dilution (0.1µg) (Red), compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) and an unlabeled control (cells without incubation with primary antibody) (Blue).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-NCAM1 antibody [EP2567Y] (AB303718)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-NCAM1 antibody [EP2567Y] (AB303718)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labeling NCAM1 with ab303718 at 1/50 dilution (10 µg/ml) (Red). Confocal image showing membranous and weak cytoplasmic staining in SH-SY5Y cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Green). The nuclear counterstain was DAPI (Blue).

関連する標識済み抗体及び組成の異なる製品 (10)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP2567Y

アイソタイプ

IgG

標識

Alexa Fluor® 594

励起波長/蛍光波長

Ex: 590nm, Em: 617nm

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

下記製品にもご興味をお持ちいただけるかもしれません:

AB315307

Human NCAM1 ELISA Kit

0

0 Reviews

製品ページを見る
こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

style
left-column

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.
See full target information NCAM1
style
left-column
style
left-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
style
left-column
style
right-column

関連製品

Select an associated product type
Alternative Version
Primary Antibodies

AB237455

Alexa Fluor® 488 Anti-NCAM1 antibody [EP2567Y]

primary-antibodies

alexa-fluor-488-ncam1-antibody-ep2567y-ab237455

0

(0 reviews)

Alternative Version
Primary Antibodies

AB215981

Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free

primary-antibodies

ncam1-antibody-ep2567y-low-endotoxin-azide-free-ab215981

0

(0 reviews)

Alternative Version
Primary Antibodies

AB214435

Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free

primary-antibodies

ncam1-antibody-ep2567y-bsa-and-azide-free-ab214435

0

(0 reviews)

Alternative Version
Primary Antibodies

AB303038

HRP Anti-NCAM1 antibody [EP2567Y]

primary-antibodies

hrp-ncam1-antibody-ep2567y-ab303038

0

(0 reviews)

Alternative Version
Primary Antibodies

AB237457

PE Anti-NCAM1 antibody [EP2567Y]

primary-antibodies

pe-ncam1-antibody-ep2567y-ab237457

0

(0 reviews)

Alternative Version
Primary Antibodies

AB237456

Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y]

primary-antibodies

alexa-fluor-647-ncam1-antibody-ep2567y-ab237456

0

(0 reviews)

Alternative Version
Primary Antibodies

AB303037

APC Anti-NCAM1 antibody [EP2567Y]

primary-antibodies

apc-ncam1-antibody-ep2567y-ab303037

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com