AB178000

Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control

Name: Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab178000)
Brand: Abcam Limited
SKU: AB178000
Price: 74000 JPY
Availability: InStock

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Mouse IgG1, kappa isotype control conjugated to Alexa Fluor® 594 - suitable for ICC/IF. Clone [15-6E10A7] is a mouse monoclonal isotype control.

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB178000)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB178000)

Immunocytochemistry data for ab178000. All panels show HeLa cells stained with DAPI as well as; a no primary control (A), ab178000 at 0.1 μg/mL (B), an Alexa 594 anti-SDHA conjugated antibody (ab170172) at 0.1 μg/mL (C).

Cells were PFA fixed and stored in 0.02%azide/PBS in 4°C, then blocked with 10%GS for 2 hours, primary was diluted in 10%GS and incubated for 2 hours, cells were washed 3x with 1%GS and DAPI was added for 10 minutes in 1%GS.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

15-6E10A7

アイソタイプ

IgG1

軽鎖のタイプ

kappa

標識

Alexa Fluor® 594

励起波長/蛍光波長

Ex: 590nm, Em: 617nm

キャリアフリー

アプリケーション

ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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製品の詳細

Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody's host species, isotype, and possible conjugation. The Control performed appropriately in all materials and platforms that were tested.

Product was previously marketed under the MitoSciences sub-brand.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Precipitation Ammonium Sulphate

バッファー組成

Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control