AB178000

Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control

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Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control Suitable for ICC/IF and reacts with samples.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB178000)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB178000)

Immunocytochemistry data for ab178000. All panels show HeLa cells stained with DAPI as well as; a no primary control (A), ab178000 at 0.1 μg/mL (B), an Alexa 594 anti-SDHA conjugated antibody (ab170172) at 0.1 μg/mL (C).

Cells were PFA fixed and stored in 0.02%azide/PBS in 4°C, then blocked with 10%GS for 2 hours, primary was diluted in 10%GS and incubated for 2 hours, cells were washed 3x with 1%GS and DAPI was added for 10 minutes in 1%GS.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

15-6E10A7

アイソタイプ

IgG1

軽鎖のタイプ

kappa

標識

Alexa Fluor® 594

励起波長/蛍光波長

Ex: 590nm, Em: 617nm

キャリアフリー

アプリケーション

ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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製品の詳細

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Precipitation Ammonium Sulphate

バッファー組成

Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

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Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control