AB208075

Alexa Fluor® 594 Anti-MEK1 + MEK2 抗体 [EPR16667]

Alexa Fluor® 594 Anti-MEK1 + MEK2 antibody [EPR16667]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal MEK1 antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

MEK1, PRKMK1, MAP2K1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-MEK1 + MEK2 antibody [EPR16667] (AB208075)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-MEK1 + MEK2 antibody [EPR16667] (AB208075)

ab208075 staining MEK1 + MEK2 in A431 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208075 at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16667

アイソタイプ

IgG

標識

Alexa Fluor® 594

励起波長/蛍光波長

Ex: 590nm, Em: 617nm

キャリアフリー

交差種

Human, Human, Mouse, Rat

アプリケーション

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "This product gave a positive signal in A431 cells fixed with 4% formaldehyde (10 min)", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MEK1 and MEK2 also known as MAP2K1 and MAP2K2 respectively are dual-specificity kinases that play roles in signaling pathways mediating cellular responses. MEK1 and MEK2 have molecular weights of approximately 45kDa. They are expressed in various tissues including the heart brain and skeletal muscles reflecting their widespread function within the organism. These proteins are often referred collectively as the MEK1/2 complex due to their shared and overlapping roles in cell signaling.

Biological function summary

MEK1 and MEK2 are integral parts of the mitogen-activated protein kinase (MAPK) signaling cascade. MEK1/2 phosphorylate and activate the ERK1/2 kinases which transmit signals from the cell surface to the nucleus regulating gene expression and cell fate decisions. The MEK1/2 proteins also act in concert with other kinases and substrates forming signaling complexes that ensure specific and regulated cellular outcomes.

Pathways

MEK1 and MEK2 play key roles within the MAPK/ERK pathway a critical signaling mechanism for cell division and survival. They also link to the RAS/MAPK pathway involved in transmitting signals from membrane receptors to the nucleus. In these pathways MEK1/2 interact with proteins like RAF kinases and ERK1/2 propagating signals that influence cellular growth and differentiation.

MEK1 and MEK2 are implicated in cancer and neurological disorders. Abnormal activity of the MEK1/2 proteins often due to mutations can lead to uncontrolled cell proliferation contributing to the development of cancers such as melanoma. In neurological disorders dysregulation of MEK1/2 signaling relates to conditions like neuro-cardio-facial-cutaneous syndromes. These proteins interact with other key players in these diseases such as mutant RAF proteins in melanoma highlighting their critical roles in disease mechanisms.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed : 29433126). Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

See full target information MAP2K1

追加のターゲット

MAP2K2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:2024 PubMed29789628

2018

Targetable vulnerabilities in T- and NK-cell lymphomas identified through preclinical models.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Y Ng,Noriaki Yoshida,Amanda L Christie,Mahmoud Ghandi,Neekesh V Dharia,Joshua Dempster,Mark Murakami,Kay Shigemori,Sara N Morrow,Alexandria Van Scoyk,Nicolas A Cordero,Kristen E Stevenson,Maneka Puligandla,Brian Haas,Christopher Lo,Robin Meyers,Galen Gao,Andrew Cherniack,Abner Louissaint,Valentina Nardi,Aaron R Thorner,Henry Long,Xintao Qiu,Elizabeth A Morgan,David M Dorfman,Danilo Fiore,Julie Jang,Alan L Epstein,Ahmet Dogan,Yanming Zhang,Steven M Horwitz,Eric D Jacobsen,Solimar Santiago,Jian-Guo Ren,Vincent Guerlavais,D Allen Annis,Manuel Aivado,Mansoor N Saleh,Amitkumar Mehta,Aviad Tsherniak,David Root,Francisca Vazquez,William C Hahn,Giorgio Inghirami,Jon C Aster,David M Weinstock,Raphael Koch

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 594 Anti-MEK1 + MEK2 抗体 [EPR16667]

Alexa Fluor® 594 Anti-MEK1 + MEK2 antibody [EPR16667]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-MEK1 + MEK2 antibody [EPR16667] (AB208075)

関連する標識済み抗体及び組成の異なる製品 (8)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (1)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise