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AB207895

Alexa Fluor® 594 Anti-IL-2 Receptor alpha 抗体 [EPR6452]

Alexa Fluor® 594 Anti-IL-2 Receptor alpha antibody [EPR6452]

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(2 Publications)

Rabbit Recombinant Monoclonal IL2RA antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.

別名を表示する

CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-IL-2 Receptor alpha antibody [EPR6452] (AB207895)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-IL-2 Receptor alpha antibody [EPR6452] (AB207895)

ab207895 staining IL2 receptor in K562 cells. The cells were fixed with 80% methanol and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab207895 at 1/167 dilution (pseudocolored in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 488), at 1/250 dilution (shown in green/red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in K562 cells fixed with 4% formaldehyde.

関連する標識済み抗体及び組成の異なる製品 (3)

  • Carrier free

    Anti-IL-2 Receptor alpha antibody [EPR6452] - BSA and Azide free

  • Unconjugated

    Anti-IL-2 Receptor alpha antibody [EPR6452]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-IL-2 Receptor alpha antibody [EPR6452]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR6452

アイソタイプ

IgG

標識

Alexa Fluor® 594

励起波長/蛍光波長

Ex: 590nm, Em: 617nm

キャリアフリー

No

交差種

Human, Human

アプリケーション

IHC-P, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/167", "ICCIF-species-notes": "<p>This product gave a positive signal in K562 cells fixed with 4% formaldehyde (10 min) and 80% methanol (5 min)</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
See full target information IL2RA

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature methods 21:2248-2259 PubMed39478175

2024

Quality control for single-cell analysis of high-plex tissue profiles using CyLinter.

Applications

Unspecified application

Species

Unspecified reactive species

Gregory J Baker,Edward Novikov,Ziyuan Zhao,Tuulia Vallius,Janae A Davis,Jia-Ren Lin,Jeremy L Muhlich,Elizabeth A Mittendorf,Sandro Santagata,Jennifer L Guerriero,Peter K Sorger

Frontiers in immunology 13:940093 PubMed36203611

2022

IL-10 partly mediates the ability of MSC-derived extracellular vesicles to attenuate myocardial damage in experimental metabolic renovascular hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Yamei Jiang,Siting Hong,Xiangyang Zhu,Lei Zhang,Hui Tang,Kyra L Jordan,Ishran M Saadiq,Weijun Huang,Amir Lerman,Alfonso Eirin,Lilach O Lerman
View all publications

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