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リコンビナントRabMAb

Alexa Fluor® 594 Anti-GFAP 抗体 [EP672Y] (ab302663)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-GFAP antibody [EP672Y] (AB302663)
  • Alexa Fluor® 594 Anti-GFAP antibody [EP672Y] (AB302663)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [EP672Y] to GFAP
  • Suitable for: ICC/IF
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 750 Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 594 Anti-GFAP antibody [EP672Y]
    GFAP 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 594 Rabbit monoclonal [EP672Y] to GFAP

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

  • アプリケーション

    適用あり: ICC/IFmore details

  • 種交差性

    交差種: Mouse
    非交差種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: mouse primary neural/glia cell

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302663の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/50.
特記事項
ICC/IF
1/50.

ターゲット情報

  • 機能

    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

  • 組織特異性

    Expressed in cells lacking fibronectin.

  • 関連疾患

    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.

  • 配列類似性

    Belongs to the intermediate filament family.

  • 翻訳後修飾

    Phosphorylated by PKN1.

  • 細胞内局在

    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt

  • 参照データベース

    • 別名

      • wu:fb34h11 antibody
      • ALXDRD antibody
      • cb345 antibody
      • etID36982.3 antibody
      • FLJ42474 antibody
      • FLJ45472 antibody
      • GFAP antibody
      • GFAP_HUMAN antibody
      • gfapl antibody
      • Glial fibrillary acidic protein antibody
      • Intermediate filament protein antibody
      • wu:fk42c12 antibody
      • xx:af506734 antibody
      • zgc:110485 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-GFAP antibody [EP672Y] (AB302663)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-GFAP antibody [EP672Y] (AB302663)

      Immunofluorescent analysis of 4% paraformaldehyde fixed and 0.1% TritonX-100 permeabilized mouse primary neural/glia cell labeling GFAP with ab302663 at 1/50 dilution (10 μg/ml). Counterstained with Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody at 1:100 (1 μg/mL) (Green). Confocal image showing positive staining in mouse primary glia cell. 

      Nuclear couterstain is DAPI (Blue).

      Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

    • Alexa Fluor® 594 Anti-GFAP antibody [EP672Y] (AB302663)
      Alexa Fluor® 594 Anti-GFAP antibody [EP672Y] (AB302663)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302663 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302663 は論文での使用が確認できていません。

    レビューと Q&A

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