AB203419

Alexa Fluor® 594 Anti-Desmin 抗体 [Y66] - Cytoskeleton Marker

Alexa Fluor® 594 Anti-Desmin antibody [Y66] - Cytoskeleton Marker

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal Desmin antibody - conjugated to Alexa Fluor® 594. Cytoskeleton marker. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

別名を表示する

Desmin, DES

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (AB203419)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (AB203419)

ab203419 staining Desmin in A673 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203419 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in A673 cells fixed with 100% methanol (5 min).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

Y66

アイソタイプ

IgG

標識

Alexa Fluor® 594

励起波長/蛍光波長

Ex: 590nm, Em: 617nm

キャリアフリー

交差種

Human, Human, Mouse, Rat

アプリケーション

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

エピトープ

ab32362 reacts with an epitope located in the C terminal region of desmin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "This product gave a positive signal in A673 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Desmin also referred to as Desmin 20 is an intermediate filament protein with a molecular weight of approximately 53 kDa. Primarily found in muscle cells desmin provides structural integrity to cells being an important component of the cytoskeleton. Desmin expression is highly specific to cardiac skeletal and smooth muscle tissues and it can be detected using desmin marker techniques like desmin IHC (Immunohistochemistry). Desmin staining or desmin stain methods are commonly employed in diagnostic labs to visualize this protein within tissue samples.

Biological function summary

Desmin plays a significant role in maintaining cellular architecture by anchoring organelles such as nuclei and mitochondria within the cytoplasm. It associates with other proteins to form desmin intermediate filament networks which facilitate force transmission and maintain mechanical integrity. This protein does not operate in isolation but is part of a larger complex of cytoskeletal elements that reinforce muscle cell shape and resilience. Its interactions and binding with other cytoskeletal components confirm its essential role in muscle structure.

Pathways

Desmin links closely to the cell structure and function pathways providing stability during muscle contraction processes. It interacts with other proteins including actin and tubulin within the cytoskeleton network ensuring coordinated responses to mechanical stress. Specifically desmin integrates into pathways related to the maintenance of the sarcomere structure thereby connecting to additional important proteins like myosin and dystrophin which collectively uphold muscular function and integrity.

Desmin mutations can lead to myopathies such as desmin-related myopathy (DRM) or cardiomyopathy where the structural disruptions result in abnormal muscle function. These conditions typically manifest as muscle weakness or cardiac abnormalities. Furthermore its relationship to the sarcomere suggests interactions with proteins like dystrophin in muscular dystrophies where desmin integrity critically influences disease progression and severity. Understanding desmin's link to such disorders underpins its role as a diagnostic marker and a potential therapeutic target in muscle-related diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed : 25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed : 24200904, PubMed : 25394388, PubMed : 26724190). May act as a sarcomeric microtubule-anchoring protein : specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Required for nuclear membrane integrity, via anchoring at the cell tip and nuclear envelope, resulting in maintenance of microtubule-derived intracellular mechanical forces (By similarity). Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).

See full target information DES

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 19:e70025 PubMed40520075

2025

Adipose-derived stem cell exosomes alleviate TGF-β1-induced urethral stricture fibrosis by suppressing the TGF-β/Smad pathway and downstream PDGFR-β/RAS/ERK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liang,Chao Deng,Hang Guo,Zhenghao Dai,Yiwen Jiang,Yuting Lu,Weiguo Chen

Cell reports 43:114925 PubMed39475511

2024

Muscle inflammation is regulated by NF-κB from multiple cells to control distinct states of wasting in cancer cachexia.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin R Pryce,Alexander Oles,Erin E Talbert,Martin J Romeo,Silvia Vaena,Sudarshana Sharma,Victoria Spadafora,Lauren Tolliver,David A Mahvi,Katherine A Morgan,William P Lancaster,Eryn Beal,Natlie Koren,Bailey Watts,Morgan Overstreet,Stefano Berto,Suganya Subramanian,Kubra Calisir,Anna Crawford,Brian Neelon,Michael C Ostrowski,Teresa A Zimmers,James G Tidball,David J Wang,Denis C Guttridge

Heliyon 10:e37227 PubMed39296007

2024

Molecular basis of CX-5461-induced DNA damage response in primary vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tengfei Liu,Guopin Pan,Jing Zhang,Jianli Wang,Xiaosun Guo,Ye Chen,Xiaoyun Wang,Xiaopei Cui,Huiqing Liu,Fan Jiang

Nature neuroscience 27:1892-1903 PubMed39210068

2024

Characteristics of blood-brain barrier heterogeneity between brain regions revealed by profiling vascular and perivascular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah J Pfau,Urs H Langen,Theodore M Fisher,Indumathi Prakash,Faheem Nagpurwala,Ricardo A Lozoya,Wei-Chung Allen Lee,Zhuhao Wu,Chenghua Gu

Brain sciences 14: PubMed38928601

2024

CD59 Protects Primary Human Cerebrovascular Smooth Muscle Cells from Cytolytic Membrane Attack Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Carson D Whinnery,Ying Nie,Danilo S Boskovic,Salvador Soriano,Wolff M Kirsch

International journal of molecular sciences 25: PubMed38256174

2024

Potential Molecular Markers Related to Lymph Node Metastasis and Stalk Resection Margins in Pedunculated T1 Colorectal Cancers Using Digital Spatial Profiling: A Pilot Study with a Small Case Series.

Applications

Unspecified application

Species

Unspecified reactive species

Mi Jung Kwon,Ha Young Park,Hyun Lim,Il Tae Son,Min-Jeong Kim,Nan Young Kim,Min Jeong Kim,Eun Sook Nam,Seong Jin Cho,Woo Jin Bang,Ho Suk Kang

Cancers 13: PubMed34199452

2021

HGF/c-Met Inhibition as Adjuvant Therapy Improves Outcomes in an Orthotopic Mouse Model of Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tony C Y Pang,Zhihong Xu,Alpha Raj Mekapogu,Srinivasa Pothula,Therese Becker,Susan Corley,Marc R Wilkins,David Goldstein,Romano Pirola,Jeremy Wilson,Minoti Apte

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 594 Anti-Desmin 抗体 [Y66] - Cytoskeleton Marker

Alexa Fluor® 594 Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (AB203419)

関連する標識済み抗体及び組成の異なる製品 (10)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (7)

Abcam product promise