AB203427

Alexa Fluor® 594 Anti-Cytokeratin 7 抗体 [EPR1619Y] - Cytoskeleton Marker

Alexa Fluor® 594 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Cytokeratin 7 antibody - conjugated to Alexa Fluor® 594. Cytoskeleton marker. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

別名を表示する

SCL, KRT7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7, CK7

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB203427)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB203427)

ab203427 staining Cytokeratin 7 in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203427 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in T47D cells fixed with 100% methanol (5 min).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR1619Y

アイソタイプ

IgG

標識

Alexa Fluor® 594

励起波長/蛍光波長

Ex: 590nm, Em: 617nm

キャリアフリー

交差種

Human, Human

アプリケーション

IHC-P, Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

See full target information KRT7

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5536 PubMed40593574

2025

Lysosome-mediated aggregation of galactose-deficient IgA1 with transferrin receptor 1 links to IgA nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Meijun Si,Jingpeng Fu,Mengting Fang,Yunfei Lu,Junxuan Huang,Haojie Li,Peiyi Wang,Maofu Liao,Jian Zhu,Peiyao Li,Wenzhao Zhong,Zhifei Guo,Wei Yang,Zhiming Ye,Hongli Hu,Xueqing Yu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 594 Anti-Cytokeratin 7 抗体 [EPR1619Y] - Cytoskeleton Marker

Alexa Fluor® 594 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB203427)

関連する標識済み抗体及び組成の異なる製品 (6)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise