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リコンビナントRabMAb

Alexa Fluor® 594 Anti-Cytokeratin 20 抗体 [EPR1622Y] (ab303597)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab303597)
  • Alexa Fluor® 594 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab303597)
  • Alexa Fluor® 594 Anti-Cytokeratin 20 antibody [EPR1622Y] (AB303597)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [EPR1622Y] to Cytokeratin 20
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free PE Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 594 Anti-Cytokeratin 20 antibody [EPR1622Y]
    Cytokeratin 20 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 594 Rabbit monoclonal [EPR1622Y] to Cytokeratin 20

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

  • アプリケーション

    適用あり: ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: HT-29 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • バッファー

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR1622Y

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab303597の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 0.2 µg/ml.
特記事項
ICC/IF
Use a concentration of 0.2 µg/ml.

ターゲット情報

  • 機能

    Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.

  • 組織特異性

    Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.

  • 配列類似性

    Belongs to the intermediate filament family.

  • 発生段階

    First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.

  • 翻訳後修飾

    Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
    Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD20 antibody
      • CK 20 antibody
      • CK-20 antibody
      • CK20 antibody
      • Cytokeratin-20 antibody
      • Cytokeratin20 antibody
      • K1C20_HUMAN antibody
      • K20 antibody
      • KA20 antibody
      • Keratin 20 antibody
      • keratin 20, type I antibody
      • keratin 21, rat, homolog of antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 20 antibody
      • Keratin-20 antibody
      • Keratin20 antibody
      • KRT 20 antibody
      • KRT 21 antibody
      • KRT20 antibody
      • KRT21 antibody
      • MGC35423 antibody
      • OTTHUMP00000164518 antibody
      • Protein IT antibody
      • type I cytoskeletal 20 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab303597)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab303597)

      AB303597 staining KRT20 in HT-29 (human colorectal adenocarcinoma cell line) cells (positive control) and MCF7 cells (negative control). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3 M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with AB303597 at 0.2 µg/ml (shown in red) and ab195883 (Alexa Fluor®488 Anti-Tubulin antibody [YOL1/34]) at 5 µg/ml (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Alexa Fluor® 594 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab303597)
      Alexa Fluor® 594 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab303597)
    • Alexa Fluor® 594 Anti-Cytokeratin 20 antibody [EPR1622Y] (AB303597)
      Alexa Fluor® 594 Anti-Cytokeratin 20 antibody [EPR1622Y] (AB303597)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab303597 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab303597 は論文での使用が確認できていません。

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