Alexa Fluor® 594 Anti-alpha Tubulin 抗体 [DM1A] - Microtubule Marker (ab195889)
Key features and details
- Alexa Fluor® 594 Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
- Isotype: IgG1
Related conjugates and formulations
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker
alpha Tubulin 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 594 Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker -
由来種
Mouse -
標識
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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エピトープ
aa 426-450 -
ポジティブ・コントロール
- ICC/IF - HeLa
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特記事項
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
DM1A -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184093)
- Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)
- Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)
- Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free (ab264493)
- Alexa Fluor® 488 Anti-Islet 1 antibody [EP4182] (ab275990)
- HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)
- FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)
- Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab195889の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/150 - 1/250.
ab178000 - Mouse monoclonal IgG1 (Alexa Fluor® 594), is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
1/150 - 1/250. ab178000 - Mouse monoclonal IgG1 (Alexa Fluor® 594), is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
配列類似性
Belongs to the tubulin family. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 7277 Human
- Entrez Gene: 22145 Mouse
- Entrez Gene: 316531 Rat
- Omim: 191110 Human
- SwissProt: P68366 Human
- SwissProt: P68368 Mouse
- SwissProt: Q5XIF6 Rat
- Unigene: 75318 Human
see all -
別名
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling Doublecortin with AB305266 at 1/50 (10.0 ug/ml) dilution. Confocal image showing cytoplasmic staining in SH-SY5Y cells. Negative control: HeLa (PMID:18312642). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: PBS instead of the primary antibody.
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
ab195889 staining alpha Tubulin in MEF1 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% PBS-Triton X-100 for 5 min then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1hr. The cells were then incubated with ab195889 at 1/250 dilution (shown in orange) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
This product gave a positive signal in 100% methanol (10 min) fixed MEF1 cells under the same testing conditions.
Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
ab195889 staining alpha Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% PBS-Triton X-100 for 5 min then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1hr. The cells were then incubated with ab195889 at 1/167 dilution (shown in psedo-color red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
This product gave a positive signal in 100% methanol (10 min) fixed HeLa cells under the same testing conditions.
Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab195889 は 6 報の論文で使用されています。
- Zheng T et al. Functional mechanism of hsa-miR-128-3p in epithelial-mesenchymal transition of pancreatic cancer cells via ZEB1 regulation. PeerJ 10:e12802 (2022). PubMed: 35186455
- Cong Y et al. Ptk2b deletion improves mice folliculogenesis and fecundity via inhibiting follicle loss mediated by Erk pathway. J Cell Physiol 236:1043-1053 (2021). PubMed: 32608523
- Otaka N et al. Vasohibin-2 Aggravates Development of Ascending Aortic Aneurysms but not Abdominal Aortic Aneurysms nor Atherosclerosis in ApoE-Deficient Mice. Am J Hypertens 34:467-475 (2021). PubMed: 33180898
- Pelisch F et al. Sumoylation regulates protein dynamics during meiotic chromosome segregation in C. elegans oocytes. J Cell Sci 132:N/A (2019). PubMed: 31243051
- Qadri MM et al. cAMP Attenuates TGF-ß's Profibrotic Responses in Osteoarthritic Synoviocytes: Involvement of Hyaluronan and PRG4. Am J Physiol Cell Physiol N/A:N/A (2018). PubMed: 29898378
- Seifert J et al. Thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy. Sci Rep 7:4810 (2017). PubMed: 28684746