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AB312497

Alexa Fluor® 568 Anti-USP9x 抗体 [EPR13809(B)] - N-terminal

Alexa Fluor® 568 Anti-USP9x antibody [EPR13809(B)] - N-terminal

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Rabbit Recombinant Monoclonal USP9X antibody - conjugated to Alexa Fluor® 568.

別名を表示する

DFFRX, FAM, USP9, USP9X, Ubiquitin carboxyl-terminal hydrolase 9X, Deubiquitinating enzyme FAF-X, Fat facets in mammals, Ubiquitin thioesterase FAF-X, Ubiquitin-specific-processing protease FAF-X, hFAM

関連する標識済み抗体及び組成の異なる製品 (9)

  • Unconjugated

    Anti-USP9x antibody [EPR13809(B)] - N-terminal

  • 578 PE

    PE Anti-USP9x antibody [EPR13809(B)] (N-terminal)

  • HRP

    HRP Anti-USP9x antibody [EPR13809(B)] (N-terminal)

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-USP9x antibody [EPR13809(B)] - N-terminal

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-USP9x antibody [EPR13809(B)] - N-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-USP9x antibody [EPR13809(B)] - N-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-USP9x antibody [EPR13809(B)] - N-terminal

  • 660 APC

    APC Anti-USP9x antibody [EPR13809(B)] (N-terminal)

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-USP9x antibody [EPR13809(B)] - N-terminal

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR13809(B)

アイソタイプ

IgG

標識

Alexa Fluor® 568

励起波長/蛍光波長

Ex: 578nm, Em: 603nm

キャリアフリー

No

アプリケーション

Target Binding Affinity, Antibody Labelling

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

USP9x also known as ubiquitin-specific peptidase 9 X-linked is a deubiquitinating enzyme that plays a critical role in protein homeostasis. USP9x belongs to the USP family and has an approximate molecular mass of 290 kDa. It is expressed broadly in human tissues with particularly high levels in the brain and pancreas. Mechanically USP9x functions to remove ubiquitin molecules from ubiquitinated substrates preventing their degradation by the proteasome. This regulatory action influences many cellular processes by stabilizing specific proteins.
Biological function summary

USP9x influences numerous cellular mechanisms by maintaining protein balance. It is a part of the complex cellular machinery involved in signal transduction cell division and apoptosis. While its enzyme activity regulates various cellular proteins USP9x is essential in neural development and differentiation. The proteolytic activity helps to modulate pathways by controlling protein turnover which impacts cellular responses and development. Failure to regulate proteins accurately can lead to compromised cellular functions.

Pathways

USP9x participates prominently in the Wnt signaling and TGF-beta pathways. Its role in deubiquitination allows it to stabilize proteins like beta-catenin and SMAD4 which are vital for transmitting signals within these pathways. Through these interactions USP9x aids in cell proliferation migration and differentiation often connecting with other proteins like APC or Axin in the Wnt pathway. This demonstrates how USP9x's enzymatic activity intricately links to key biological signaling mechanisms.

Mutations and dysregulation of USP9x associate with cancer and neurodevelopmental disorders. In cancer it impairs normal cell cycle control and apoptosis facilitating unchecked cell proliferation. It interacts with proteins such as MCL1 influencing apoptosis decisions and tumor growth. In neurodevelopmental disorders USP9x affects brain development by disrupting neural cell differentiation processes. The protein's malfunction causes imbalances in brain signaling linking it to conditions like intellectual disability.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (PubMed : 18254724, PubMed : 19135894, PubMed : 22371489, PubMed : 25944111, PubMed : 29626158, PubMed : 30914461, PubMed : 37454738). May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin (PubMed : 18254724, PubMed : 19135894, PubMed : 22371489, PubMed : 25944111, PubMed : 29626158, PubMed : 30914461, PubMed : 37454738). Specifically hydrolyzes 'Lys-11'-, followed by 'Lys-63'-, 'Lys-48'- and 'Lys-6'-linked polyubiquitins chains (PubMed : 30914461). Essential component of TGF-beta/BMP signaling cascade (PubMed : 19135894). Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33 (PubMed : 19135894). Deubiquitinates alkylation repair enzyme ALKBH3 (PubMed : 25944111). OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions (PubMed : 25944111). Deubiquitinates RNA demethylase enzyme ALKBH5, promoting its stability (PubMed : 37454738). Deubiquitinates mTORC2 complex component RICTOR at 'Lys-294' by removing 'Lys-63'-linked polyubiquitin chains, stabilizing RICTOR and enhancing its binding to MTOR, thus promoting mTORC2 complex assembly (PubMed : 33378666). Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres (PubMed : 16322459). Involved in axonal growth and neuronal cell migration (PubMed : 24607389). Regulates cellular clock function by enhancing the protein stability and transcriptional activity of the core circadian protein BMAL1 via its deubiquitinating activity (PubMed : 29626158). Acts as a regulator of peroxisome import by mediating deubiquitination of PEX5 : specifically deubiquitinates PEX5 monoubiquitinated at 'Cys-11' following its retrotranslocation into the cytosol, resetting PEX5 for a subsequent import cycle (PubMed : 22371489). Deubiquitinates PEG10 (By similarity). Inhibits the activation of the Hippo signaling pathway via deubiquitination of AMOTL2 at 'Lys-347' and 'Lys-408' which prohibits its interaction with and activation of LATS2. Loss of LATS2 activation and subsequent loss of YAP1 phosphorylation results in an increase in YAP1-driven transcription of target genes (PubMed : 26598551, PubMed : 34404733).
See full target information USP9X
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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。
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関連製品

Select an associated product type
Alternative Version
Primary Antibodies

AB250191

Anti-USP9x antibody [EPR13809(B)] - BSA and Azide free

primary-antibodies

usp9x-antibody-epr13809b-bsa-and-azide-free-ab250191

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