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AB312371

Alexa Fluor® 568 Anti-Rab5 抗体 [EPR21801] - Early Endosome Marker

Alexa Fluor® 568 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

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Rabbit Recombinant Monoclonal RAB5A antibody - conjugated to Alexa Fluor® 568. Early Endosome marker.

別名を表示する

RAB5, RAB5A, Ras-related protein Rab-5A

関連する標識済み抗体及び組成の異なる製品 (8)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 578 PE

    PE Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 660 APC

    APC Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • HRP

    HRP Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Rab5 antibody [EPR21801]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR21801

アイソタイプ

IgG

標識

Alexa Fluor® 568

励起波長/蛍光波長

Ex: 578nm, Em: 603nm

キャリアフリー

No

アプリケーション

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody could cross-react with RAB5C, but affinity is very low.

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Rab5 also known as Ras-related protein Rab-5A is a small GTPase of approximately 24 kDa. It is part of the Rab family of proteins and functions as an endosomal marker in cells. Rab5 is ubiquitously expressed but shows enriched expression in tissues with a high rate of endocytosis like the brain and liver. This protein plays a significant role in early endosome fusion and trafficking acting as an essential regulator of vesicular transport processes within the cell.
Biological function summary

Rab5 regulates the early endocytic pathway by mediating vesicle docking and fusion processes. It is not a part of a large complex but interacts with a range of effector proteins that facilitate endosome maturation. Rab5's role as an endosomal marker makes it important for membrane trafficking receptor recycling and signal transduction. Researchers often identify Rab5 in cellular studies using endosome staining techniques or specific antibodies like Drosophila antibodies to visualize its function and distribution.

Pathways

The endocytic network heavily involves Rab5 particularly influencing the endocytosis and endosomal signaling pathways. It frequently interacts with proteins such as EEA1 (Early Endosome Antigen 1) and Rabaptin-5 which are critical for endosomal membrane fusion. This mechanistic involvement ensures the correct transfer and processing of endocytic vesicles facilitating the continual cycle of membrane and receptor turnover in cells.

Rab5 dysfunction associates with neurological conditions such as Alzheimer's disease and cancer. Alterations in Rab5 levels or function can disrupt normal endosomal trafficking leading to amyloid beta peptide accumulation in Alzheimer's. Additionally Rab5's role in cancer relates to its regulation of growth factor receptors where its interaction with proteins like the PE marker influences tumor progression. Understanding Rab5 pathways and interactions paves the way for developing targeted therapeutics in these disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed : 10818110, PubMed : 14617813, PubMed : 15378032, PubMed : 16410077). Contributes to the regulation of filopodia extension (PubMed : 14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed : 22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).
See full target information RAB5A
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