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AB207282

Alexa Fluor® 568 Anti-NeuN 抗体 [EPR12763] - Neuronal Marker

Alexa Fluor® 568 Anti-NeuN antibody [EPR12763] - Neuronal Marker

5

(1 Review)

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(10 Publications)

Anti-NeuN antibody [EPR12763] - Neuronal Marker - Alexa Fluor® 568 conjugated (ab207282) is a rabbit recombinant monoclonal antibody detecting NeuN in ICC/IF. Suitable for Rat.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Neuronal nuclei antigen, NeuN antigen, RBFOX3

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB207282)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB207282)

ab207282 staining NeuN in B35 cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207282 at 1/100 dilution (pseudocolored in green) and ab190573, Rabbit monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

This product also gave a positive signal under the same testing conditions in B35 cells fixed with 100% methanol (5 min).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB207282)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB207282)

ab207282 staining NeuN in PC12 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207282 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in PC12 cells fixed with 4% formaldehyde (10 min).

関連する標識済み抗体及び組成の異なる製品 (8)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker

  • 519 FITC

    FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-NeuN antibody [EPR12763] - Neuronal Marker

  • Biotin

    Biotin Anti-NeuN antibody [EPR12763] - Neuronal Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-NeuN antibody [EPR12763] - Neuronal Marker

  • Unconjugated

    Anti-NeuN antibody [EPR12763] - Neuronal Marker

  • Carrier free

    Anti-NeuN antibody [EPR12763] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR12763

アイソタイプ

IgG

標識

Alexa Fluor® 568

励起波長/蛍光波長

Ex: 578nm, Em: 603nm

キャリアフリー

No

交差種

Rat, Human, Mouse, Rat

アプリケーション

Flow Cyt (Intra), ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

What is this antibody validated in?
Alexa Fluor® 568 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207282) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Rat samples.

Related products
Antibody clone EPR12763 is also available pre-conjugated to a variety of labels for your convenience – Anti-NeuN Alexa Fluor® 568 [EPR12763] - Neuronal Marker (ab207282).

Other related products
We have a range of other formats of antibody clone [EPR12763] also available for your convenience: ab177487, Alexa Fluor® 488 - ab190195, Alexa Fluor® 647 - ab190565, Biotin - ab204681, Alexa Fluor® 594 - ab207279, Alexa Fluor® 555 - ab207281, Alexa Fluor® 568 - ab207282, Carrier free - ab209898, FITC - ab223994, ab279295, ab279296, ab279297, Carrier free - ab279307, Carrier free - ab279308, Carrier free - ab279309

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NeuN also known as Fox-3 or Rbfox3 serves as a vital neuronal marker and has a molecular weight of about 46-50 kDa. This nuclear protein is commonly found in neuronal cells in the central nervous system but it does not appear in glial cells. Researchers use NeuN staining as an effective tool to identify neurons in various brain regions due to its reliable expression pattern in mature neurons. The presence of NeuN can be detected using methods such as NeuN IHC (immunohistochemistry) and NeuN Western blot aiding in cellular and tissue analysis.
Biological function summary

NeuN plays an essential role in the regulation of RNA-binding influencing the splicing and stability of transcripts. It forms part of the complex network responsible for managing neuron-specific gene expression. By interacting with other RNA-binding proteins NeuN contributes to the fine-tuning needed for proper neuronal development and function. As a result NeuN helps maintain the health and activity of nerve cells allowing them to perform complex neurological tasks.

Pathways

NeuN has a significant role in neuronal differentiation and plasticity pathways. NeuN coordinates with elements of the Notch signaling pathway which is important for guiding cell fate decisions in the nervous system. It also interacts with related proteins like Fox proteins involved in splicing regulation. These pathways ensure neurons develop differentiate and function correctly within the nervous system's precise architecture.

Disruptions in NeuN expression link closely to neurodegenerative diseases such as Parkinson's disease and Amyotrophic lateral sclerosis (ALS). Abnormal NeuN expression correlates with neuron loss and dysfunction in these conditions with connectivity to other affected proteins like alpha-synuclein in Parkinson's disease. In ALS abnormal NeuN-staining patterns indicate neuronal cell death serving as a potential marker for disease progression. Studying these connections improves our understanding of disease mechanisms and aids in the development of targeted therapies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
See full target information NeuN
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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 39:5099-5111 PubMed39092980

2024

Acute Neurobehavioral and Glial Responses to Explosion Gas Inhalation in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jinren Liu,Junhong Gao,Hong Wang,Xiaolin Fan,Liang Li,Xiangni Wang,Xiying Wang,Jiajia Lu,Xingmin Shi,Pinglin Yang

eLife 12: PubMed36656755

2023

Tissue libraries enable rapid determination of conditions that preserve antibody labeling in cleared mouse and human tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Theodore J Zwang,Rachel E Bennett,Maria Lysandrou,Benjamin Woost,Anqi Zhang,Charles M Lieber,Douglas S Richardson,Bradley T Hyman

Progress in neurobiology 223:102386 PubMed36481386

2022

Tau promotes oxidative stress-associated cycling neurons in S phase as a pro-survival mechanism: Possible implication for Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Marine Denechaud,Sarah Geurs,Thomas Comptdaer,Séverine Bégard,Alejandro Garcia-Núñez,Louis-Adrien Pechereau,Thomas Bouillet,Yannick Vermeiren,Peter P De Deyn,Romain Perbet,Vincent Deramecourt,Claude-Alain Maurage,Michiel Vanderhaegen,Sebastiaan Vanuytven,Bruno Lefebvre,Elke Bogaert,Nicole Déglon,Thierry Voet,Morvane Colin,Luc Buée,Bart Dermaut,Marie-Christine Galas

Nature biomedical engineering 7:520-532 PubMed36192597

2022

Ultraflexible electrode arrays for months-long high-density electrophysiological mapping of thousands of neurons in rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengtuo Zhao,Hanlin Zhu,Xue Li,Liuyang Sun,Fei He,Jason E Chung,Daniel F Liu,Loren Frank,Lan Luan,Chong Xie

Nature neuroscience 25:285-294 PubMed35210624

2022

Clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Ratz,Leonie von Berlin,Ludvig Larsson,Marcel Martin,Jakub Orzechowski Westholm,Gioele La Manno,Joakim Lundeberg,Jonas Frisén

Frontiers in neuroanatomy 15:752234 PubMed34867215

2021

Comparison of Different Tissue Clearing Methods for Three-Dimensional Reconstruction of Human Brain Cellular Anatomy Using Advanced Imaging Techniques.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Scardigli,Luca Pesce,Niamh Brady,Giacomo Mazzamuto,Vladislav Gavryusev,Ludovico Silvestri,Patrick R Hof,Christophe Destrieux,Irene Costantini,Francesco S Pavone

Nature communications 12:4518 PubMed34312393

2021

9-Cyanopyronin probe palette for super-multiplexed vibrational imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Yupeng Miao,Naixin Qian,Lixue Shi,Fanghao Hu,Wei Min

Journal of neural engineering 16:035001 PubMed30736013

2019

Parallel, minimally-invasive implantation of ultra-flexible neural electrode arrays.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengtuo Zhao,Xue Li,Fei He,Xiaoling Wei,Shengqing Lin,Chong Xie

Neuroscience 388:45-56 PubMed30030056

2018

Chemogenetic Manipulation of Dorsal Hippocampal Astrocytes Protects Against the Development of Stress-enhanced Fear Learning.

Applications

Unspecified application

Species

Unspecified reactive species

Meghan E Jones,Jacqueline E Paniccia,Christina L Lebonville,Kathryn J Reissner,Donald T Lysle

Brain, behavior, and immunity 67:355-363 PubMed28963000

2017

Hippocampal interleukin-1 mediates stress-enhanced fear learning: A potential role for astrocyte-derived interleukin-1β.

Applications

Unspecified application

Species

Unspecified reactive species

Meghan E Jones,Christina L Lebonville,Jacqueline E Paniccia,Megan E Balentine,Kathryn J Reissner,Donald T Lysle
View all publications
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代替製品

Primary Antibodies

AB104225

Anti-NeuN antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB104225)

  • 5
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Primary Antibodies

AB134014

Anti-NeuN antibody - Neuronal Marker

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB134014)

  • 2
  • 9
Primary Antibodies

AB128886

Anti-NeuN antibody - Neuronal Marker

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB128886)

  • 5
  • 14
Primary Antibodies

AB236870

Anti-NeuN antibody [EPR21906] - Neuronal Marker

Advanced Validation|RabMAb|Recombinant|Lab Essentials|20ul selling size|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21906] - Neuronal Marker (AB236870)

  • 5
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Primary Antibodies

AB239345

Anti-NeuN antibody [EPR21902] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21902] - BSA and Azide free (AB239345)

  • 0
  • 0
Primary Antibodies

AB239347

Anti-NeuN antibody [EPR21906] - BSA and Azide free

Advanced Validation|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)

  • 0
  • 0

組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
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Abcam product promise

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