Alexa Fluor® 568 Anti-MAP2 抗体 [EPR19691]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB303465)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue stained for MAP2 using ab303465 at a 1/100 dilution (5.0 μg/ml) (Red). Positive staining on human cerebrum. The section was incubated with ab303455 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB303465)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MAP2 with ab303465 at 1/100 dilution (5.0 µg/mL). Positive staining is observed on human cerebrum. The section was incubated with ab303465 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins was used.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB303465)

Immunofluorescence staining of MAP2 staining in a section of formalin-fixed paraffin-embedded human Alzheimer’s brain*.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (Ph9.0) using retrieval settings of 100°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab303465 at 1/500 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB303465)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue stained for MAP2 using ab303465 at a 1/100 dilution (5.0 μg/ml) (Red). Positive staining on rat cerebrum. The section was incubated with ab303455 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB303465)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue stained for MAP2 using ab303465 at a 1/100 dilution (5.0 μg/ml) (Red). Positive staining on mouse cerebrum. The section was incubated with ab303455 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB303465)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling MAP2 with ab303465 at 1/100 dilution (5.0 µg/mL). Positive staining is observed on mouse cerebrum. The section was incubated with ab303465 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins was used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB303465)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling MAP2 with ab303465 at 1/100 dilution (5.0 µg/mL). Positive staining is observed on rat cerebrum. The section was incubated with ab303465 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins was used.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB303465)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labeling MAP2 with ab303465 at 1/250 dilution (2 μg/ml) (Red). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 μg/ml dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Green). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB303465)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labeling MAP2 with ab303465 at 1/250 dilution (2 ug/ml) (Green). Confocal image showing positive staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm, followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 μg/ml dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Green). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB303465)

Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neurons staining MAP2 using ab303465 at a 1/250 dilution (2 μg/ml) (Red). Counterstained with ab11267 Anti-MAP2 mouse monoclonal antibody at a 1/500 dilution (4 μg/ml), followed by ab50113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution (2 μg/ml) (Green). Nuclear counterstain is DAPI (Blue). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB303465)

Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neurons staining MAP2 using ab303465 at a 1/250 dilution (2 μg/ml) (Red). Counterstained with ab11267 Anti-MAP2 mouse monoclonal antibody at a 1/500 dilution (4 μg/ml), followed by ab50113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution (2 μg/ml) (Green). Nuclear counterstain is DAPI (Blue). Confocal image showing positive staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.