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AB312595

Alexa Fluor® 568 Anti-Ku70 抗体 [EPR4027]

Alexa Fluor® 568 Anti-Ku70 antibody [EPR4027]

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Rabbit Recombinant Monoclonal Ku70 antibody - conjugated to Alexa Fluor® 568.

別名を表示する

G22P1, XRCC6, X-ray repair cross-complementing protein 6, 5'-deoxyribose-5-phosphate lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, DNA repair protein XRCC6, Lupus Ku autoantigen protein p70, Thyroid-lupus autoantigen, X-ray repair complementing defective repair in Chinese hamster cells 6, 5'-dRP lyase Ku70, CTC75, CTCBF, Ku70, TLAA

関連する標識済み抗体及び組成の異なる製品 (10)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4027

アイソタイプ

IgG

標識

Alexa Fluor® 568

励起波長/蛍光波長

Ex: 578nm, Em: 603nm

キャリアフリー

No

アプリケーション

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Ku70 also known as XRCC6 or K331 is a DNA repair protein with a molecular mass of approximately 70 kDa. It belongs to the Ku protein family which includes Ku80. This protein expresses mainly in the nucleus of cells across various tissue types. As a part of a heterodimer together with Ku80 Ku70 forms the Ku70/Ku80 complex which binds to free DNA ends allowing DNA repair processes to occur efficiently.
Biological function summary

The Ku70 protein plays a significant role in maintaining genomic stability. It is an important component of the non-homologous end joining (NHEJ) pathway a primary mechanism for repairing double-strand breaks in DNA. By forming the Ku70/Ku80 complex it accurately aligns DNA ends to facilitate repair and prevent chromosomal abnormalities. The Ku70 protein also involves in telomere maintenance and can influence cell cycle regulation.

Pathways

Ku70 contributes mainly to the NHEJ DNA repair pathway. This pathway is important for cellular response to DNA damage and involves other proteins such as DNA-PKcs (DNA-dependent protein kinase catalytic subunit) and XRCC4. Ku70's interaction with DNA-PKcs enables the activation of its kinase activity which is essential for the repair process. Additionally Ku70 is involved in the homologous recombination pathway where it interacts with proteins like Rad51 to ensure repair fidelity.

Research links the Ku70 protein to cancer and neurodegenerative diseases. Dysregulation or mutations affecting Ku70 can lead to genomic instability contributing to cancer development. Ku70 also shows connections to neurodegenerative conditions like Alzheimer's disease where its role in DNA repair and apoptosis regulation may impact neuronal cell survival. Interactions with p53 a protein important for DNA damage response further underline Ku70's influence in these disease contexts.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). Required for double-strand break repair and V(D)J recombination (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). Also has a role in chromosome translocation (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). Has a role in chromosome translocation (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). It works in the 3'-5' direction (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step : it recognizes and binds to the broken ends of the DNA and protects them from further resection (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). Binding to DNA may be mediated by XRCC6 (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). The XRCC5-XRRC6 dimer acts as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks (PubMed : 20383123). 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined (PubMed : 20383123). The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed : 8621488). In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed : 12145306). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed : 28712728).
See full target information XRCC6
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