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AB313089

Alexa Fluor® 568 Anti-CD8 抗体 [SP239]

Alexa Fluor® 568 Anti-CD8 antibody [SP239]

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Rabbit Recombinant Monoclonal CD8 alpha antibody - conjugated to Alexa Fluor® 568.

別名を表示する

CD8a, MAL, CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

関連する標識済み抗体及び組成の異なる製品 (7)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP239

アイソタイプ

IgG

標識

Alexa Fluor® 568

励起波長/蛍光波長

Ex: 578nm, Em: 603nm

キャリアフリー

No

アプリケーション

Target Binding Affinity, Antibody Labelling

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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下記製品にもご興味をお持ちいただけるかもしれません:

AB256393

Human CD8A ELISA Kit

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0 Reviews

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こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD8 also known as CD8a or Leu-2 is a glycoprotein with a molecular weight of approximately 32 kDa. It is prominently expressed on the surface of cytotoxic T lymphocytes. These CD8 molecules facilitate the interaction between T cells and antigen-presenting cells by binding to major histocompatibility complex (MHC) class I molecules. This interaction is essential for the immune system to recognize and destroy infected or cancerous cells. CD8 is often identified using various markers like CD8 FITC CD8 APC and CD8 PerCP which are used in flow cytometry for cell sorting and analysis.
Biological function summary

CD8 plays a significant role in the adaptive immune response. It is not part of a multimeric complex but functions as a co-receptor that enhances the recognition of antigens by the T cell receptor (TCR). The presence of CD8 molecules aids the activation of T cells leading to their proliferation and the effective targeting and elimination of pathogens or abnormal cells. The ability of CD8 to bind to MHC class I molecules allows cytotoxic T cells to detect and respond to nearly all nucleated cells in the body.

Pathways

CD8 is integral in both the T cell receptor signaling pathway and the induction of apoptosis. In the T cell receptor signaling pathway CD8 works closely with other proteins like Lck and ZAP-70 to transmit signals upon TCR-MHC class I engagement. This promotes T cell activation cytokine production and cell division. Furthermore CD8's interaction with Fas ligand pathways contributes to the initiation of programmed cell death which is important for maintaining immune homeostasis and preventing overreaction of the immune system.

CD8 T cells are significant in the context of viral infections and autoimmune diseases. For example during HIV infection the depletion of CD8 T cells impairs the immune response allowing the virus to evade the immune system. Additionally in autoimmune disorders like Type 1 diabetes CD8 T cells mistakenly target and destroy insulin-producing beta cells in the pancreas. These pathological processes also involve proteins such as MHC class I and other immune modulators like interleukins that modulate CD8 T cell activity and differentiation.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

The protein expressed by the gene CD8A is an integral membrane glycoprotein crucial for immune responses, operating mainly in T-cells as a coreceptor for MHC class I molecule : peptide complexes. These peptides originate from cytosolic proteins, unlike class II peptides, which are from extracellular proteins. CD8A interacts with the T-cell receptor (TCR) and MHC class I proteins on antigen-presenting cells, recruiting the Src kinase LCK to the TCR-CD3 complex. LCK phosphorylates substrates, triggering signaling pathways that lead to the production of lymphokines, enhanced motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs), thereby aiding in the recognition and elimination of infected or tumor cells. Additionally, in natural killer (NK) cells, CD8A homodimers on the cell surface provide a survival mechanism for conjugating with and lysing multiple target cells. CD8A homodimers also facilitate the survival and differentiation of activated lymphocytes into memory CD8 T-cells. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CD8A
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Abcam product promise

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