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AB312456

Alexa Fluor® 568 Anti-Aly 抗体 [EPR17942]

Alexa Fluor® 568 Anti-Aly antibody [EPR17942]

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Rabbit Recombinant Monoclonal Aly/Ref antibody - conjugated to Alexa Fluor® 568.

別名を表示する

ALY, BEF, THOC4, ALYREF, THO complex subunit 4, Tho4, Ally of AML-1 and LEF-1, Aly/REF export factor, Transcriptional coactivator Aly/REF, bZIP-enhancing factor BEF

関連する標識済み抗体及び組成の異なる製品 (10)

  • Unconjugated

    Anti-Aly/Ref antibody [EPR17942]

  • 578 PE

    PE Anti-Aly/Ref antibody [EPR17942]

  • 660 APC

    APC Anti-Aly/Ref antibody [EPR17942]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Aly antibody [EPR17942]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Aly antibody [EPR17942]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Aly antibody [EPR17942]

  • HRP

    HRP Anti-Aly/Ref antibody [EPR17942]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Aly antibody [EPR17942]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Aly/Ref antibody [EPR17942]

  • Carrier free

    Anti-Aly/Ref antibody [EPR17942] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR17942

アイソタイプ

IgG

標識

Alexa Fluor® 568

励起波長/蛍光波長

Ex: 578nm, Em: 603nm

キャリアフリー

No

アプリケーション

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Aly/Ref also known as THOC4 is a protein that plays an important role in mRNA processing and transport. It is a member of the TREX (transcription/export) complex having a molecular mass of approximately 27 kDa. Aly/Ref is primarily expressed in the nucleus where it binds to spliced mRNAs and ensures their export to the cytoplasm. Functionally Aly/Ref interacts with mRNA export adapters attaching to the cap-binding complex at the 5' end of mRNA facilitating its journey to the nuclear pore.
Biological function summary

Aly/Ref functions by forming tight associations with the TREX complex which includes other proteins like UAP56. It is responsible for coupling transcription with mRNA processing and export helping to maintain the integrity of gene expression. Aly/Ref directly influences the efficiency and accuracy of mRNA export impacting gene regulation by ensuring that only fully processed transcripts exit the nucleus. By doing so it helps coordinate the transcription and mRNA export processes.

Pathways

Aly/Ref associates with the mRNA surveillance pathway and the RNA export pathway. Aly/Ref works closely with proteins like EJC components which are involved in checking mRNA quality before export. This participation ensures that only correctly spliced mRNAs move from the nucleus to the cytoplasm where they translate into proteins. Aly/Ref also contributes to maintaining cellular homeostasis by regulating the amount of mRNA available for translation.

Aly/Ref relates to cancer and neurological disorders. Changes in the expression or function of Aly/Ref can disrupt mRNA export leading to abnormal protein synthesis which is associated with cancer development. Furthermore Aly/Ref interacts with proteins such as NXF1 which are implicated in the onset of certain neurological disorders when mutated or misregulated revealing its broader impact on cellular health and disease progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Functions as an mRNA export adapter; component of the transcription/export (TREX) complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed : 15833825, PubMed : 15998806, PubMed : 17190602). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed : 15833825, PubMed : 15998806, PubMed : 17190602). Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B (PubMed : 17984224). Plays a key role in mRNP recognition and mRNA packaging by bridging the mRNP-bound EJC and the TREX core complex (PubMed : 37020021). TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1 (PubMed : 15833825, PubMed : 15998806, PubMed : 17190602, PubMed : 37020021). Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC) (PubMed : 15998806, PubMed : 17984224, PubMed : 37020021). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway) (PubMed : 11675789, PubMed : 11707413, PubMed : 11979277, PubMed : 15833825, PubMed : 15998806, PubMed : 17190602, PubMed : 18364396, PubMed : 22144908, PubMed : 22893130, PubMed : 23222130, PubMed : 25662211). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim (PubMed : 19165146). Involved in mRNA export of C5-methylcytosine (m5C)-containing mRNAs : specifically recognizes and binds m5C mRNAs and mediates their nucleo-cytoplasmic shuttling (PubMed : 28418038). Acts as a chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation (PubMed : 10488337). Involved in transcription elongation and genome stability (PubMed : 12438613).. (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA.
See full target information ALYREF
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関連製品

Select an associated product type
Alternative Version
Primary Antibodies

AB240364

Anti-Aly/Ref antibody [EPR17942] - BSA and Azide free

primary-antibodies

aly-ref-antibody-epr17942-bsa-and-azide-free-ab240364

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