AB320065

Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease 抗体 [GT-38] - Conformation-Specific

Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific

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Mouse Monoclonal TAU antibody - conjugated to Alexa Fluor® 555. Suitable for IHC-Fr and reacts with Human samples.

別名を表示する

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

2 Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (AB320065)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (AB320065)

Negative immunofluorescence staining of Tau Alzheimer’s Disease in a section of frozen human normal brain.

The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND^TM. The section was incubated at room temperature for 1 hour with ab320065 at 1/250 dilution. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (AB320065)

Immunofluorescence staining of Tau Alzheimer’s Disease in a section of frozen human Alzheimer brain.

The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND^TM. The section was incubated at room temperature for 1 hour with ab320065 at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

GT-38

アイソタイプ

IgG1

標識

Alexa Fluor® 555

励起波長/蛍光波長

Ex: 555nm, Em: 565nm

キャリアフリー

交差種

Human

アプリケーション

IHC-Fr

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/250", "IHCFr-species-notes": "<p></p>" } } }

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製品の詳細

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tau protein also known as MAPT (Microtubule-Associated Protein Tau) plays a mechanical role in stabilizing microtubules in neuronal axons. The human tau protein has several isoforms generally weighing between 45 to 65 kDa. Tau is predominantly expressed in neurons of the central nervous system but can also be found in some non-neuronal cells. In neurons tau binds to tubulin and facilitates the polymerization and stability of microtubules which are essential for maintaining cellular structure and transport functions.

Biological function summary

Tau protein impacts the dynamics of the neuronal cytoskeleton. Tau is part of the intracellular transport system where it facilitates the movement of organelles and proteins along axons. This function is essential for neuron communication and resilience. Tau interacts with and regulates microtubule-associated complexes ensuring efficient intracellular transport. Its role in cytoskeletal integrity strongly influences cell signaling pathways critical for synaptic plasticity and neuronal survival.

Pathways

Tau protein plays a significant part in cellular transport and signal transduction pathways. One key pathway is the MAPK signaling pathway where tau's phosphorylation state influences neuronal response to external stimuli. Additionally tau is involved in the PI3K/AKT pathway affecting cell growth and survival. Within these pathways tau's phosphorylation is regulated by kinases including GSK-3β and CDK5 while phosphatases like PP2A dephosphorylate tau to maintain its functional state.

Dysfunctional tau is strongly linked to neurodegenerative diseases particularly Alzheimer's disease (AD) and frontotemporal dementia (FTD). In AD hyperphosphorylated tau forms neurofibrillary tangles which disrupt neuronal function and promote cell death. This process often involves other proteins such as beta-amyloid which exacerbate tau's pathological aggregation. Understanding tauopathy mechanisms has guided the research and development of anti-tau antibodies like anti-GT3 and anti-conformation antibodies as therapeutic strategies against tau-related pathologies in neurodegenerative disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease 抗体 [GT-38] - Conformation-Specific

Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (AB320065)

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (AB320065)

関連する標識済み抗体及び組成の異なる製品 (5)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise