AB207014

Alexa Fluor® 555 Anti-STAT6 抗体 [YE361]

Alexa Fluor® 555 Anti-STAT6 antibody [YE361]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal STAT6 antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications.

別名を表示する

Signal transducer and activator of transcription 6, IL-4 Stat, STAT6

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-STAT6 antibody [YE361] (AB207014)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-STAT6 antibody [YE361] (AB207014)

ab207014 staining STAT6 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207014 at a 1/100 dilution (shown in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor^® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-STAT6 antibody [YE361] (AB207014)

ab207014 staining STAT6 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207014 at a 1/100 dilution (shown in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor^® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

YE361

アイソタイプ

IgG

標識

Alexa Fluor® 555

励起波長/蛍光波長

Ex: 555nm, Em: 565nm

キャリアフリー

交差種

Human

アプリケーション

ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.

Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Carries out a dual function : signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

See full target information STAT6

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102154 PubMed38511173

2024

STAT6-targeting antisense oligonucleotides against solitary fibrous tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Li,Jose L Mondaza-Hernandez,David S Moura,Alexey S Revenko,Angelica Tolentino,John T Nguyen,Nam Tran,Clark A Meyer,Jose Merino-Garcia,Rafael Ramos,Davide Di Lernia,Javier Martin-Broto,Heather N Hayenga,Leonidas Bleris

Nature communications 14:7479 PubMed37980418

2023

Comprehensive analysis reveals potential therapeutic targets and an integrated risk stratification model for solitary fibrous tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Renjing Zhang,Yang Yang,Chunfang Hu,Mayan Huang,Wenjian Cen,Dongyi Ling,Yakang Long,Xin-Hua Yang,Boheng Xu,Junling Peng,Sujie Wang,Weijie Zhu,Mingbiao Wei,Jiaojiao Yang,Yuxia Xu,Xu Zhang,Jiangjun Ma,Fang Wang,Hongtu Zhang,Peiqing Ma,Xiaojun Zhu,Guohui Song,Li-Yue Sun,De-Shen Wang,Feng-Hua Wang,Yu-Hong Li,Sandro Santagata,Qin Li,Yan-Fen Feng,Ziming Du

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 555 Anti-STAT6 抗体 [YE361]

Alexa Fluor® 555 Anti-STAT6 antibody [YE361]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-STAT6 antibody [YE361] (AB207014)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-STAT6 antibody [YE361] (AB207014)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

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