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リコンビナントRabMAb

Alexa Fluor® 555 Anti-Rb 抗体 [EPR17512] (ab300672)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Rb antibody [EPR17512] (ab300672)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-Rb antibody [EPR17512] (AB300672)
  • Alexa Fluor® 555 Anti-Rb antibody [EPR17512] (AB300672)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EPR17512] to Rb
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 555 Anti-Rb antibody [EPR17512]
    Rb 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 555 Rabbit monoclonal [EPR17512] to Rb

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

  • アプリケーション

    適用あり: ICC/IF, Flow Cyt (Intra)more details
    適用なし: IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: MCF7 (human breast adenocarcinoma epithelial cell). Flow cyt. Intr.: MCF7 (human breast adenocarcinoma epithelial cell).

  • 特記事項

    ab300672 does not react in IHC-P with human and mouse species. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300672の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/50.
Flow Cyt (Intra)
1/50.
特記事項
ICC/IF
1/50.
Flow Cyt (Intra)
1/50.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

  • 組織特異性

    Expressed in the retina.

  • 関連疾患

    Childhood cancer retinoblastoma
    Bladder cancer
    Osteogenic sarcoma

  • 配列類似性

    Belongs to the retinoblastoma protein (RB) family.

  • ドメイン

    The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.

  • 翻訳後修飾

    Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
    N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
    Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Exon 17 tumor GOS561 substitution mutation causes premature stop antibody
      • GOS563 exon 17 substitution mutation causes premature stop antibody
      • OSRC antibody
      • Osteosarcoma antibody
      • p105-Rb antibody
      • P105RB antibody
      • PP105 antibody
      • pp110 antibody
      • PPP1R130 antibody
      • pRb antibody
      • Prepro retinoblastoma associated protein antibody
      • Protein phosphatase 1 regulatory subunit 130 antibody
      • Rb antibody
      • RB transcriptional corepressor 1 antibody
      • RB_HUMAN antibody
      • RB1 antibody
      • RB1 gene antibody
      • Retinoblastoma 1 antibody
      • Retinoblastoma suspectibility protein antibody
      • Retinoblastoma-associated protein antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Rb antibody [EPR17512] (ab300672)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Rb antibody [EPR17512] (ab300672)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling Rb with ab300672 at 1/50 dilution (10.0 µg/mL) (Red). Confocal image showing nuclear staining in MCF7 cells. ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Green). The nuclear counterstain was DAPI (Blue).

       

    • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-Rb antibody [EPR17512] (AB300672)
      Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-Rb antibody [EPR17512] (AB300672)

      Flow cytometric (Intracellular) analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cells) labeling Rb with ab300672 at 1/50 dilution (1 µg) (Red) compared with a Rabbit monoclonal isotype control - Alexa Fluor®555 (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    • Alexa Fluor® 555 Anti-Rb antibody [EPR17512] (AB300672)
      Alexa Fluor® 555 Anti-Rb antibody [EPR17512] (AB300672)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300672 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300672 は論文での使用が確認できていません。

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