Alexa Fluor® 555 Anti-Olig2 抗体 [EPR2673] (ab300730)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Olig2 antibody [EPR2673] (ab300730)](https://www.abcam.co.jp/ps/products/300/ab300730/Images/AB300730-1-anti-olig2-antibody-epr2673-immunocytochemistry-rat-primary-neuroglia-cell1.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Olig2 antibody [EPR2673] (ab300730)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 555 Rabbit monoclonal [EPR2673] to Olig2
- Suitable for: ICC/IF
- Reacts with: Rat
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 555 Anti-Olig2 antibody [EPR2673]
Olig2 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 555 Rabbit monoclonal [EPR2673] to Olig2 -
由来種
Rabbit -
標識
Alexa Fluor® 555. Ex: 555nm, Em: 565nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC/IF: Rat primary neural/glia cells
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2673 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300730の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/50.
|
| 特記事項 |
|---|
|
ICC/IF
1/50. |
ターゲット情報
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機能
Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. -
組織特異性
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. -
関連疾患
Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
ドメイン
The bHLH is essential for interaction with NKX2-2. -
細胞内局在
Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2. - Information by UniProt
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参照データベース
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別名
- Basic domain helix loop helix protein class B 1 antibody
- Basic helix loop helix protein class B 1 antibody
- BHLHB antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Olig2 antibody [EPR2673] (ab300730)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cells labelling Olig2 with ab300730 at 1/50 dilution (10.0 µg/mL) (Red). Confocal image showing positive staining in rat primary neural/glia cells. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 μg/ml) (Green). The Nuclear counterstain was DAPI (Blue).
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![Alexa Fluor® 555 Anti-Olig2 antibody [EPR2673] (AB300730)](https://www.abcam.co.jp/ps/products/300/ab300730/Images/AB300730-2-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 555 Anti-Olig2 antibody [EPR2673] (AB300730)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab300730 は論文での使用が確認できていません。