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AB313206

Alexa Fluor® 555 Anti-Nestin 抗体 [SP103]

Alexa Fluor® 555 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

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Rabbit Recombinant Monoclonal Nestin antibody - conjugated to Alexa Fluor® 555.

別名を表示する

Nbla00170, NES, Nestin

関連する標識済み抗体及び組成の異なる製品 (9)

  • 660 APC

    APC Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • 578 PE

    PE Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Nestin antibody [SP103]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • Unconjugated

    Anti-Nestin antibody [SP103] - Neural Stem Cell Marker

  • Carrier free

    Anti-Nestin antibody [SP103] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP103

アイソタイプ

IgG

標識

Alexa Fluor® 555

励起波長/蛍光波長

Ex: 555nm, Em: 565nm

キャリアフリー

No

アプリケーション

Target Binding Affinity, Antibody Labelling

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Nestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.
Biological function summary

Nestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.

Pathways

Nestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.

Nestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
See full target information NES
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組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB190565

Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB190565)

  • 5
  • 7
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92

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