Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker 抗体 [EPR18709]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (AB307768)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling LC3B with ab307768 at 1/100 dilution (5.0 μg/ml).

Negative control : no staining on human stomach.

The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (AB307768)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labeling LC3B with ab307768 at 1/50 dilution (10 μg/ml) (Red).

Confocal image showing increased cytoplasmic staining in HeLa cells treated with Chloroquine (50μM) for 24 hours.

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml) (Green). The nuclear counterstain was DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (AB307768)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling LC3B with ab307768 at 1/100 dilution (5.0 μg/ml).

Cytoplasmic staining on human cerebrum.

The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (AB307768)

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling LC3B with ab307768 at 1/100 dilution (5.0 μg/ml).
Cytoplasmic staining on human glioma.
The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).