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リコンビナントRabMAb

Alexa Fluor® 555 Anti-Huntingtin 抗体 [EPR5526] (ab302550)

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Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Huntingtin antibody [EPR5526] (AB302550)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Huntingtin antibody [EPR5526] (AB302550)
  • Alexa Fluor® 555 Anti-Huntingtin antibody [EPR5526] (AB302550)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EPR5526] to Huntingtin
  • Suitable for: IHC-Fr
  • Reacts with: Mouse, Rat
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 555 Anti-Huntingtin antibody [EPR5526]
    Huntingtin 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 555 Rabbit monoclonal [EPR5526] to Huntingtin

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

  • 特異性

    AB302550 does not react in immunocytochemistry with human and mouse species. 

  • アプリケーション

    適用あり: IHC-Frmore details
    適用なし: ICC

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-Fr.: Rat and mouse cerebellum (fresh) frozen tissue.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • バッファー

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR5526

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302550の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
1/50.
特記事項
IHC-Fr
1/50.
追加情報
Is unsuitable for ICC.

ターゲット情報

  • 機能

    May play a role in microtubule-mediated transport or vesicle function.

  • 組織特異性

    Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.

  • 関連疾患

    Defects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).

  • 配列類似性

    Belongs to the huntingtin family.
    Contains 10 HEAT repeats.

  • ドメイン

    The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.

  • 翻訳後修飾

    Cleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
    Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.

  • 細胞内局在

    Cytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AI256365 antibody
      • C430023I11Rik antibody
      • HD antibody
      • HD protein antibody
      • HD_HUMAN antibody
      • HDH antibody
      • HTT antibody
      • Huntingtin antibody
      • HUNTINGTON CHOREA antibody
      • Huntington disease protein antibody
      • Huntington's disease protein homolog antibody
      • IT 15 antibody
      • IT15 antibody
      • OTTMUSP00000026909 antibody
      • ZHD antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Huntingtin antibody [EPR5526] (AB302550)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Huntingtin antibody [EPR5526] (AB302550)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum (fresh) tissue labeling Huntingtin with ab302550 at 1/50 (10.0 μg/ml) dilution (Red). Confocal image showing positive staining on mouse cerebellum. The nuclear counterstain was DAPI (Blue). Negative control: Primary diluent was used instead of primary antibody.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Huntingtin antibody [EPR5526] (AB302550)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Huntingtin antibody [EPR5526] (AB302550)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum (fresh) tissue labeling Huntingtin with ab302550 at 1/50 (10.0 μg/ml) dilution (Red). Confocal image showing positive staining on rat cerebellum. The nuclear counterstain was DAPI (Blue). Negative control: Primary diluent was used instead of primary antibody.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 555 Anti-Huntingtin antibody [EPR5526] (AB302550)
      Alexa Fluor® 555 Anti-Huntingtin antibody [EPR5526] (AB302550)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302550 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302550 は論文での使用が確認できていません。

    レビューと Q&A

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