Alexa Fluor® 555 Anti-GFAP 抗体 [EPR1034Y]

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB201735)

IHC image of GFAP staining in a section of frozen normal rat dentate gyrus.

The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab201735 at 1/100 (pseudocolored in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB201735)

Image A : composite multiplex immunohistochemistry staining using conjugates panel composed of anti-P2Y12 (Alexa Fluor^® 488) (ab309607, 1/50 dilution, 10 µg/mL) (green), anti-GFAP (Alexa Fluor^® 555) (ab201735, 1/100 dilution, 5 µg/mL) (magenta) and anti-NeuN (Alexa Fluor^® 647) (ab190565, 1/100 dilution, 5 µg/mL) (white) on Frozen sections of mouse cerebellum. Nuclear DNA was labelled with DAPI (blue).

Image B : Frozen section of mouse cerebellum stained with anti- P2Y12 (Alexa Fluor^® 488) (ab309607, 1/50 dilution, 10 µg/mL).

Image C : Frozen section of mouse cerebellum stained with anti-GFAP (Alexa Fluor^® 555) (ab201735, 1/100 dilution, 5 µg/mL).

Image D : Frozen section of mouse cerebellum stained with anti-NeuN (Alexa Fluor^® 647) (ab190565, 1/100 dilution, 5 µg/mL).

Immunohistochemistry staining was performed on a Leica Biosystems BOND^® RX instrument. The section was initially fixed using 10% formaldehyde in 1XPBS for 10 minutes. The section was then incubated at room temperature for 1 hour and mounted using Dako Fluorescence Mounting Medium^®.

Image acquisition was performed with Leica SP8 confocal microscope. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB201735)

Image A : composite multiplex immunohistochemistry staining using conjugates panel composed of anti-NeuN (Alexa Fluor^® 488) (ab190195, 1/100 dilution, 5 µg/mL) (green), anti-GFAP (Alexa Fluor^® 555) (ab201735, 1/100 dilution, 5 µg/mL) (magenta) and anti-Synaptophysin (Alexa Fluor^® 647) (ab196166, 1/100 dilution, 5 µg/mL) (white) on Formalin/PFA-fixed paraffin-embedded sections of rat cerebellum. Nuclear DNA was labelled with DAPI (blue).

Image B : Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-NeuN (Alexa Fluor^® 488) (ab190195, 1/100 dilution, 5 µg/mL).

Image C : Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-GFAP (Alexa Fluor^® 555) (ab201735, 1/100 dilution, 5 µg/mL).

Image D : Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti-Synaptophysin (Alexa Fluor^® 647) (ab196166, 1/100 dilution, 5 µg/mL).

Immunohistochemistry staining was performed on a Leica Biosystems BOND^® RX instrument. Heat mediated antigen retrieval with EDTA (pH 9.0, 110°C) for 40 mins. The section was then incubated at room temperature for 1 hour and mounted using Dako Fluorescence Mounting Medium^®.

Image acquisition was performed with Leica SP8 confocal microscope. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.