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AB318901

Alexa Fluor® 555 Anti-CCR6 抗体 [EPR22259]

Alexa Fluor® 555 Anti-CCR6 antibody [EPR22259]

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Rabbit Recombinant Monoclonal CCR6 antibody - conjugated to Alexa Fluor® 555.

別名を表示する

CD196, CKRL3, CMKBR6, GPR29, STRL22, CCR6, C-C chemokine receptor type 6, C-C CKR-6, CC-CKR-6, CCR-6, Chemokine receptor-like 3, DRY6, G-protein coupled receptor 29, GPR-CY4, LARC receptor, CKR-L3, GPRCY4

関連する標識済み抗体及び組成の異なる製品 (6)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22259

アイソタイプ

IgG

標識

Alexa Fluor® 555

励起波長/蛍光波長

Ex: 555nm, Em: 565nm

キャリアフリー

No

アプリケーション

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CCR6 also known as CD196 is a chemokine receptor with a molecular mass of approximately 44 kDa. This protein is primarily expressed on the surface of various immune cells including T cells B cells and dendritic cells. Through its interaction with its only known ligand CCL20 also known as MIP-3α CCR6 mediates chemotaxis which guides the targeted migration of cells to sites of inflammation or injury. In murine models (mouse) CCR6 expression helps researchers examine immune responses in vivo and its presence is detected using recombinant proteins or antibodies specific for CCR6 such as 'CCR6 antibodies' with techniques including flow cytometry utilizing labels like CCR6-APC or CCR6-fluorochrome.
Biological function summary

CCR6 plays an essential role in the regulation of immune responses and maintaining mucosal immunity. It is involved in the recruitment of Th17 cells and dendritic cells to inflamed tissues which plays a critical role in initiating and sustaining immune responses. While CCR6 operates primarily as a standalone receptor its engagement influences diverse cellular functions linked to immune surveillance and tolerance. This receptor's expression on lymphoid tissue inducer cells indicates its relevance in the formation of secondary lymphoid organs and in dynamic interactions within the immune system.

Pathways

CCR6 is a significant component in pathways related to immune cell trafficking and inflammatory responses particularly the MAPK/ERK pathway and the JAK/STAT pathway. In these pathways CCR6 often interacts with related proteins such as CCL20 and other chemokine receptors facilitating cellular migration and activation processes. These interactions underpin the migration of regulatory T cells and B cells which modulate inflammation and affect pathogen clearance ensuring a controlled immune response.

CCR6 has links to conditions such as autoimmune diseases with rheumatoid arthritis and inflammatory bowel disease being notable examples. In rheumatoid arthritis the dysregulated expression of CCR6 attracts Th17 cells into synovial tissues promoting inflammation. Meanwhile inflammatory bowel disease shows a similar pattern of CCR6-mediated chemotaxis that encourages Th17 cell recruitment. In these contexts CCR6 cooperates with other immune mediators like CCL20 to perpetuate disease-related inflammatory processes highlighting its potential as a therapeutic target in immune modulation.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Receptor for the C-C type chemokine CCL20 (PubMed : 9169459). Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels (PubMed : 20068036). Although CCL20 is its major ligand it can also act as a receptor for non-chemokine ligands such as beta-defensins (PubMed : 25585877). Binds to defensin DEFB1 leading to increase in intracellular calcium ions and cAMP levels. Its binding to DEFB1 is essential for the function of DEFB1 in regulating sperm motility and bactericidal activity (PubMed : 25122636). Binds to defensins DEFB4 and DEFB4A/B and mediates their chemotactic effects (PubMed : 20068036). The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/ memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCR6-mediated signals are essential for immune responses to microbes in the intestinal mucosa and in the modulation of inflammatory responses initiated by tissue insult and trauma (PubMed : 21376174). CCR6 is essential for the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for the normal migration of Th17 cells in Peyers-patches and other related tissue sites of the intestine and plays a role in regulating effector T-cell balance and distribution in inflamed intestine. Plays an important role in the coordination of early thymocyte precursor migration events important for normal subsequent thymocyte precursor development, but is not required for the formation of normal thymic natural regulatory T-cells (nTregs). Required for optimal differentiation of DN2 and DN3 thymocyte precursors. Essential for B-cell localization in the subepithelial dome of Peyers-patches and for efficient B-cell isotype switching to IgA in the Peyers-patches. Essential for appropriate anatomical distribution of memory B-cells in the spleen and for the secondary recall response of memory B-cells (By similarity). Positively regulates sperm motility and chemotaxis via its binding to CCL20 (PubMed : 23765988).
See full target information CCR6
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組合せ製品

Proteins & Peptides

AB314615

Recombinant Human CCR6 Protein (His Tag)

SDS-PAGE - Recombinant Human CCR6 Protein (His Tag) (AB314615)

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