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AB319842

Alexa Fluor® 555 Anti-C11B2/CYP11B2 抗体 [EPR10494]

Alexa Fluor® 555 Anti-C11B2/CYP11B2 antibody [EPR10494]

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Rabbit Recombinant Monoclonal C11B2/CYP11B2 antibody - conjugated to Alexa Fluor® 555.

別名を表示する

Aldosterone synthase, Aldosterone-synthesizing enzyme, CYPXIB2, Cytochrome P-450Aldo, Cytochrome P-450C18, Steroid 18-hydroxylase, ALDOS, CYP11B2

関連する標識済み抗体及び組成の異なる製品 (6)

  • 660 APC

    APC Anti-C11B2/CYP11B2 antibody [EPR10494]

  • 578 PE

    PE Anti-C11B2/CYP11B2 antibody [EPR10494]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-C11B2/CYP11B2 antibody [EPR10494]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-C11B2/CYP11B2 antibody [EPR10494]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-C11B2/CYP11B2 antibody [EPR10494]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-C11B2/CYP11B2 antibody [EPR10494]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR10494

アイソタイプ

IgG

標識

Alexa Fluor® 555

励起波長/蛍光波長

Ex: 555nm, Em: 565nm

キャリアフリー

No

アプリケーション

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

C11B2 also known as CYP11B2 is a highly specific enzyme that plays an essential role in the biosynthesis of aldosterone. Also referred to as aldosterone synthase this cytochrome P450 family member is encoded by the CYP11B2 gene. It has a molecular mass of approximately 58 kDa. Its expression occurs mainly in the adrenal cortex particularly within the zona glomerulosa cells where it catalyzes the final steps in the production of aldosterone from cholesterol.
Biological function summary

CYP11B2 converts 11-deoxycorticosterone to corticosterone which is then converted to aldosterone. This enzyme functions independently and is not part of any larger protein complexes. It undertakes its role in the mitochondria of adrenal gland cells where it serves as an important factor in the corticosteroid synthesis pathway. Its activity is regulated via several factors including angiotensin II and potassium ion concentration to maintain homeostatic balance within the body.

Pathways

CYP11B2 is an integral part of the steroidogenic pathway specifically within the branch that leads to mineralocorticoid biosynthesis. This pathway involves the conversion of cholesterol to steroid hormones and is closely linked to the renin-angiotensin-aldosterone system (RAAS) which governs blood pressure and fluid balance. Besides its relationship with cholesterol side-chain cleavage enzyme (CYP11A1) CYP11B2 interacts with other proteins like 3-beta-hydroxysteroid dehydrogenase in facilitating steroidogenesis.

Abnormalities in CYP11B2 expression or function can lead to aldosterone-related conditions such as primary aldosteronism and Addison’s disease. Primary aldosteronism results from overproduction of aldosterone causing hypertension and electrolyte imbalances while its deficiency contributes to Addison's disease. These disorders can also involve connections with proteins like CYP17A1 which is another key player in steroid biosynthesis and whose disruptions can influence CYP11B2's activity or expression amplifying disease pathology.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

A cytochrome P450 monooxygenase that catalyzes the biosynthesis of aldosterone, the main mineralocorticoid in the human body responsible for salt and water homeostasis, thus involved in blood pressure regulation, arterial hypertension, and the development of heart failure (PubMed : 11856349, PubMed : 12530636, PubMed : 1518866, PubMed : 15356073, PubMed : 1594605, PubMed : 1775135, PubMed : 22446688, PubMed : 23322723, PubMed : 9814482, PubMed : 9814506). Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone (21-hydroxyprogesterone), namely 11-beta hydroxylation, followed by two successive oxidations at C18 yielding 18-hydroxy and then 18-oxo intermediates (that would not leave the enzyme active site during the consecutive hydroxylation reactions), ending with the formation of aldosterone (PubMed : 11856349, PubMed : 12530636, PubMed : 1518866, PubMed : 1594605, PubMed : 1775135, PubMed : 22446688, PubMed : 23322723, PubMed : 9814506). Can also produce 18-hydroxycortisol and 18-oxocortisol, derived from successive oxidations of cortisol at C18, normally found at very low levels, but significantly increased in primary aldosteronism, the most common form of secondary hypertension (PubMed : 15356073, PubMed : 9814482). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed : 11856349, PubMed : 1594605, PubMed : 23322723, PubMed : 9814506). Could also be involved in the androgen metabolic pathway (Probable).
See full target information Cytochrome P450 11B2, mitochondrial
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組合せ製品

Proteins & Peptides

AB224858

Recombinant Human C11B2/CYP11B2 protein (His tag)

SDS-PAGE - Recombinant Human C11B2/CYP11B2 protein (His tag) (AB224858)

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