Alexa Fluor® 555 Anti-c-Fos 抗体 [EPR21930-238] (ab302541)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-c-Fos antibody [EPR21930-238] (ab302541)](https://www.abcam.co.jp/ps/products/302/ab302541/Images/AB302541-488671-anticfos-antibody-epr21930-238-immunocytochemistry-hela-fbs-50.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-c-Fos antibody [EPR21930-238] (ab302541)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 555 Rabbit monoclonal [EPR21930-238] to c-Fos
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 555 Anti-c-Fos antibody [EPR21930-238]
c-Fos 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 555 Rabbit monoclonal [EPR21930-238] to c-Fos -
由来種
Rabbit -
標識
Alexa Fluor® 555. Ex: 555nm, Em: 565nm -
アプリケーション
適用あり: ICC/IFmore details
適用なし: Flow Cyt (Intra) -
種交差性
交差種: Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20% fetal bovine serum for 2 hours.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR21930-238 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302541の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/50.
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| 特記事項 |
|---|
|
ICC/IF
1/50. |
ターゲット情報
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機能
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. -
配列類似性
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain. -
翻訳後修飾
Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2353 Human
- Omim: 164810 Human
- SwissProt: P01100 Human
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別名
- Activator protein 1 antibody
- AP 1 antibody
- C FOS antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-c-Fos antibody [EPR21930-238] (ab302541)
Immunofluorescent analysis of 4% paraformaldehyde fixed and 0.1% TritonX-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labeling c-FOS with ab302541 at 1/50 dilution (10 μg/ml).
Secondary counterstain is ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488) at 1/200 dilution (shown in green).
Confocal image showing nuclear staining in HeLa cells in serum free medium for 36 hours, followed by addition of 20% fetal bovine serum for 2 hours (PMID: 14981092).
Nuclear counter satin is DAPI.
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![Alexa Fluor® 555 Anti-c-Fos antibody [EPR21930-238] (AB302541)](https://www.abcam.co.jp/ps/products/302/ab302541/Images/AB302541-1-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 555 Anti-c-Fos antibody [EPR21930-238] (AB302541)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab302541 は論文での使用が確認できていません。