AB225497

Alexa Fluor® 488 Anti-TMEM119 抗体 [106-6]

Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker

RabMAb
Recombinant
KO Validated
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(4 Publications)

Anti-TMEM119 antibody [106-6] - Alexa Fluor® 488 conjugated (ab225497) is a rabbit recombinant monoclonal antibody detecting TMEM119 in Flow Cytometry (Intra). Suitable for Human, Mouse.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

Transmembrane protein 119, Osteoblast induction factor, OBIF, Tmem119

2 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker (AB225497)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker (AB225497)

Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225497 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor^® 488 ab199091 (left). Cells were surface-stained with ab225497, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor^® 647 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

Flow Cyt (Intra)

Collaborator

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker (AB225497)

Flow cytometric analysis of wild type (WT) versus Tmem119 knockout (KO) CD11b+ cells labeling TMEM119 with ab225497 at 10 μg/mL (green) or the same clone unconjugated (ab210405) at 1 μg/mL with an Alexa 488 secondary antibody (purple), compared with TMEM119 KO primary mouse brain cells stained with ab210405 at 1 μg/mL (black) and ab225497 at 10 μg/mL (grey). Goat anti-Rabbit IgG (Alexa Fluor^®488) at 1 : 1000 dilution was used as the secondary antibody.

No signal was detected on the surface of CD11b+ brain cells from TMEM119 KO mouse stained with either the unconjugated ab210405 (black) or conjugated ab225497 antibodies (grey); whereas in wildtype CD11b+ brain cells, cell surface staining was observed (green, Alexa conjugated ab225497; purple, unconjugated ab210405 plus secondary antibody).

This image is courtesy of an anonymous collaborator.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

106-6

アイソタイプ

IgG

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

交差種

Mouse, Human, Human, Mouse

アプリケーション

ICC/IF, Flow Cyt, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "The cellular localisation of this product has been verified in ICC/IF.This antibody may be used at 10 μg/mL.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "The cellular localisation of this product has been verified in ICC/IF.This antibody may be used at 10 μg/mL.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human, Mouse samples.

Related products
Antibody clone 106-6 is also available pre-conjugated to a variety of labels for your convenience – Anti-TMEM119 Alexa Fluor® 488 [106-6] (ab225497).

Specificity confirmed
The specificity of Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497) has been confirmed by Flow Cytometry (Intracellular) testing in Tmem119 Knockout Mouse CD11b+ cells samples.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TMEM119 also known as transmembrane protein 119 plays a role in cell membrane dynamics. It has a molecular mass of approximately 35 kDa. TMEM119 is specifically expressed in microglia in the central nervous system. Microglia serve essential functions in brain homeostasis and immune response. Given its expression profile TMEM119 serves as a marker to identify microglia especially in research focused on neurological tissues. Researchers often utilize techniques like microglial flow cytometry and microglial staining for identification and quantification of microglial populations often in frozen marker studies.

Biological function summary

TMEM119 regulates signaling pathways that maintain microglial identity and function but does not typically form part of larger complexes. It modulates microglial activation states and influences responses to central nervous system injury and inflammation. As a membrane protein it may interact with other surface molecules although specific binding partners are not well-characterized in current literature. Researchers value TMEM119 as a reliable marker in studies aiming to distinguish brain-resident microglia from infiltrating peripheral macrophages.

Pathways

TMEM119 functions as a modulator within inflammatory and neuroimmune pathways. It plays roles in the NF-kB signaling and the neuroinflammatory cascade. Its relationship with proteins like CD68 and Iba1 indicates it may influence pathways tied to immune responses and phagocytosis within nervous system contexts. Understanding the interactions and co-expression of TMEM119 with these proteins helps clarify its contribution to neuroimmune functions.

TMEM119 links with neurological conditions such as Alzheimer's disease and multiple sclerosis. Altered expression of TMEM119 appears in disease states potentially contributing to pathogenesis through disrupted inflammatory responses. Its association with proteins such as TREM2 in Alzheimer's suggests that TMEM119 may play indirect roles in the progression of neurodegeneration. Furthermore high TMEM119 levels in pathological samples provide insights into microglial involvement in disease progression and potential targets for therapeutic interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays an important role in bone formation and normal bone mineralization (PubMed : 20025746, PubMed : 22416756, PubMed : 26207632). Promotes the differentiation of myoblasts into osteoblasts (PubMed : 20025746, PubMed : 22416756, PubMed : 22579779). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway (PubMed : 21239498, PubMed : 22579779). Up-regulates the expression of ATF4 which plays a central role in osteoblast differentiation (PubMed : 24362451). Essential for normal spermatogenesis and late testicular differentiation (PubMed : 26207632).

See full target information Tmem119

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40806295

2025

Induced Microglial-like Cells Derived from Familial and Sporadic Alzheimer's Disease Peripheral Blood Monocytes Show Abnormal Phagocytosis and Inflammatory Response to PSEN1 E280A Cholinergic-like Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Viviana Soto-Mercado,Miguel Mendivil-Perez,Carlos Velez-Pardo,Marlene Jimenez-Del-Rio

Cell reports 44:115176 PubMed39842435

2025

The microglial response to inhibition of Colony-stimulating-factor-1 receptor by PLX3397 differs by sex in adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Linh H D Le,Sophia Eliseeva,Elizabeth Plunk,Kallam Kara-Pabani,Herman Li,Felix Yarovinsky,Ania K Majewska

Translational stroke research 16:655-671 PubMed38558012

2024

Development of a 3D Brain Model to Study Sex-Specific Neuroinflammation After Hemorrhagic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Rezwanul Islam,Hadi Hasan Choudhary,Hritik Mehta,Feng Zhang,Tudor G Jovin,Khalid A Hanafy

Antioxidants (Basel, Switzerland) 10: PubMed34573024

2021

The Role of Endoplasmic Reticulum in the Differential Endurance against Redox Stress in Cortical and Spinal Astrocytes from the Newborn SOD1 Mouse Model of Amyotrophic Lateral Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Cecilia Marini,Vanessa Cossu,Mandeep Kumar,Marco Milanese,Katia Cortese,Silvia Bruno,Grazia Bellese,Sonia Carta,Roberta Arianna Zerbo,Carola Torazza,Matteo Bauckneht,Consuelo Venturi,Stefano Raffa,Anna Maria Orengo,Maria Isabella Donegani,Silvia Chiola,Silvia Ravera,Patrizia Castellani,Silvia Morbelli,Gianmario Sambuceti,Giambattista Bonanno

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-TMEM119 抗体 [106-6]

Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker (AB225497)

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker (AB225497)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

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Abcam product promise