AB196379

Alexa Fluor® 488 Anti-Synaptophysin 抗体 [YE269]

Name: Alexa Fluor® 488 Anti-Synaptophysin 抗体 [YE269] (ab196379) | Abcam
Brand: Abcam Limited
SKU: AB196379
Price: 89000 JPY
Availability: InStock
Rating: 4 (4 reviews)

Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] - Synaptic Marker

RabMAb
Recombinant
詳細を見る

(4 Reviews)

(4 Publications)

Rabbit Recombinant Monoclonal Synaptophysin antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 4 publications.

別名を表示する

Synaptophysin, Major synaptic vesicle protein p38, SYP

2 Images

Flow Cytometry - Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB196379)

Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB196379)

Overlay histogram showing PC12 cells stained with ab196379 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196379, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This antibody gave a positive signal in PC12 fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB196379)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB196379)

ab196379 staining Synaptophysin in NGF-differentiated PC-12 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196379 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

YE269

アイソタイプ

IgG

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

交差種

Rat, Human, Mouse, Rat

アプリケーション

IHC-P, ICC/IF, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/alexa-fluor-488-rabbit-igg-monoclonal-epr25a-isotype-control-ab199091'>ab199091</a> - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.

Biological function summary

Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.

Pathways

Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.

Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

See full target information SYP

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 28:1622-1634 PubMed40670683

2025

Evidence for trans-synaptic propagation of oligomeric tau in human progressive supranuclear palsy.

Applications

Unspecified application

Species

Unspecified reactive species

Robert I McGeachan,Lois Keavey,Elizabeth M Simzer,Ya Yin Chang,Jamie L Rose,Maxwell P Spires-Jones,Mollie Gilmore,Kristjan Holt,Soraya Meftah,Natalia Ravingerova,Cristina Scutariu,Lewis W Taylor,Declan King,Makis Tzioras,Jane Tulloch,Sam A Booker,Imran Liaquat,Nicole Hindley-Pollock,Bethany Geary,Colin Smith,Paul M Brennan,Claire S Durrant,Tara L Spires-Jones

Molecular therapy. Nucleic acids 36:102506 PubMed40206656

2025

AAV-mediated GBA1 and GDNF rescue neurological defects in a murine model of neuronopathic Gaucher disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Meng,Jiale Zhang,Ruoyue Fan,Wei Pang,Wanyang Zeng,Qingguo Guo,Xuefei Han,Ying Liu,Guangzuo Luo

Cell reports. Medicine 4:101175 PubMed37652017

2023

Human astrocytes and microglia show augmented ingestion of synapses in Alzheimer's disease via MFG-E8.

Applications

Unspecified application

Species

Unspecified reactive species

Makis Tzioras,Michael J D Daniels,Caitlin Davies,Paul Baxter,Declan King,Sean McKay,Balazs Varga,Karla Popovic,Madison Hernandez,Anna J Stevenson,Jack Barrington,Elizabeth Drinkwater,Julia Borella,Rebecca K Holloway,Jane Tulloch,Jonathan Moss,Clare Latta,Jothy Kandasamy,Drahoslav Sokol,Colin Smith,Veronique E Miron,Ragnhildur Thóra Káradóttir,Giles E Hardingham,Christopher M Henstridge,Paul M Brennan,Barry W McColl,Tara L Spires-Jones

Molecular and cellular neurosciences 88:231-239 PubMed29454667

2018

PTEN expression in astrocytic processes after spinal cord injury.

Applications

ICC/IF

Species

Unspecified reactive species

T V Povysheva,Y O Mukhamedshina,A A Rizvanov,Y A Chelyshev

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Synaptophysin 抗体 [YE269]

Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] - Synaptic Marker

Flow Cytometry - Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB196379)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB196379)

関連する標識済み抗体及び組成の異なる製品 (6)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise