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リコンビナントRabMAb

Alexa Fluor® 488 Anti-SRSF3 抗体 [EPR16976] (ab223455)

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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SRSF3 antibody [EPR16976] (ab223455)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SRSF3 antibody [EPR16976] (ab223455)
  • Alexa Fluor® 488 Anti-SRSF3 antibody [EPR16976] (ab223455)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR16976] to SRSF3
  • Suitable for: Flow Cyt (Intra), ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 750 Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 488 Anti-SRSF3 antibody [EPR16976]
    SRSF3 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 488 Rabbit monoclonal [EPR16976] to SRSF3

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR16976

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab223455の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/50.
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)
特記事項
Flow Cyt (Intra)
1/50.
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)

ターゲット情報

  • 機能

    May be involved in RNA processing in relation with cellular proliferation and/or maturation.

  • 配列類似性

    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.

  • 翻訳後修飾

    Extensively phosphorylated on serine residues in the RS domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • arginine/serine-rich 3 antibody
      • Pre mRNA splicing factor SRP20 antibody
      • Pre-mRNA-splicing factor SRP20 antibody
      • Serine/arginine-rich splicing factor 3 antibody
      • Splicing factor antibody
      • Splicing factor arginine/serine rich 20 kD antibody
      • Splicing factor arginine/serine rich 3 antibody
      • SRp20 antibody
      • SRSF3 antibody
      • SRSF3_HUMAN antibody
      • X16 antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SRSF3 antibody [EPR16976] (ab223455)
      Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SRSF3 antibody [EPR16976] (ab223455)

      Overlay histogram showing HeLa cells stained with ab223455 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223455, 1/50 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SRSF3 antibody [EPR16976] (ab223455)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SRSF3 antibody [EPR16976] (ab223455)

      ab223455 staining SRSF3 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223455 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 488 Anti-SRSF3 antibody [EPR16976] (ab223455)
      Alexa Fluor® 488 Anti-SRSF3 antibody [EPR16976] (ab223455)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab223455 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab223455 は論文での使用が確認できていません。

    レビューと Q&A

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