AB154473

Alexa Fluor® 488 Anti-SDHA 抗体 [2E3GC12FB2AE2]

Name: Alexa Fluor® 488 Anti-SDHA 抗体 [2E3GC12FB2AE2] (ab154473) | Abcam
Brand: Abcam Limited
SKU: AB154473
Price: 84000 JPY
Availability: InStock

Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2]

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(2 Publications)

Anti-SDHA antibody [2E3GC12FB2AE2] conjugated to Alexa Fluor® 488 - suitable for intracellular flow cytometry, ICC/IF, and reacts with human samples. Clone [2E3GC12FB2AE2] is a mouse monoclonal antibody cited in 6 publications.

別名を表示する

SDH2, SDHF, SDHA, Flavoprotein subunit of complex II, Malate dehydrogenase [quinone] flavoprotein subunit, Fp

4 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473)

Immunocytochemistry with HeLa cells (100x) were stained with anti-SDHA Alexa-488 antibody (1.0 µg/mL, ab154473) in green and DAPI in blue, as a nuclear stain.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473)

ab154473 staining SDHA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab154473 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473)

Flow Cytometry for Anti-SDHA Alexa-488 antibody (ab154473) stained HeLa cells. Flow cytometry results of unstained cells (black) and 0.2 µg/mL anti-SDHA Alexa-488 (red) stained cells.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473)

Immunocytochemistry with HDFn (100x) cells were stained with Anti-SDHA Alexa-488 antibody (1.0 µg/mL, ab154473) in green, Anti-HSP60 (1/1000, ab46798) as red, and DAPI in blue, as a nuclear stain. Secondary antibody used was goat anti-rabbit dyelight-594 (1/1000, ab96897).

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

2E3GC12FB2AE2

アイソタイプ

IgG1

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.2 µg/mL", "FlowCytIntra-species-notes": "<p><a href='/products/unavailable/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-alexa-fluorreg-488-ab171463'>ab171463</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

精製に関する特記事項

Purity is near homogeneity as judged by SDS-PAGE. ab154473 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

バッファー組成

Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Succinate dehydrogenase complex flavoprotein subunit A (SDHA) also known as complex II Fp or SDH2 plays an important role in the mitochondrial electron transport chain and the tricarboxylic acid (TCA) cycle. It functions as a flavoprotein oxidoreductase catalyzing the oxidation of succinate to fumarate. With a molecular mass of approximately 72 kDa SDHA is expressed in the inner mitochondrial membrane of eukaryotic cells where it is a core component of the succinate dehydrogenase complex (SDHC). The complex is essential for cellular respiration and energy production.

Biological function summary

SDHA participates in the TCA cycle by accepting electrons from succinate which it donates to the coenzyme Q in the electron transport chain. This essential role connects SDHA to the regulation of ATP production in cells. SDHA operates as part of the larger succinate dehydrogenase (SDH) complex which includes other subunits such as SDHB SDHC and SDHD. This structurally integrated multisubunit complex influences mitochondrial integrity and cellular energy homeostasis.

Pathways

SDHA is deeply involved in the TCA cycle and oxidative phosphorylation pathway. As a part of these pathways it links to other critical enzymes such as fumarase and aconitase working in concert to drive the conversion of biochemical fuel into usable cellular energy. Its interactions with coenzyme Q and cytochrome complex enzymes are important for electron flow and proton gradient formation across the mitochondrial membrane. Such interactions are central to cellular respiration and energy generation.

Mutations in SDHA correlate with various mitochondrial diseases and cancer syndromes. Specifically SDHA mutations have an association with Leigh syndrome and certain types of mitochondrial complex II deficiency. These mutations disrupt the function of the SDH complex causing metabolic imbalances and energy production issues. Furthermore the integral interaction of SDHA with other SDH subunits means that alterations can impact this entire enzymatic complex with implications for cellular respiration and disease progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed : 10746566, PubMed : 24781757). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). Can act as a tumor suppressor (PubMed : 20484225).

See full target information SDHA

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Immune network 25:e16 PubMed40620417

2025

Succinate Dehydrogenase Regulates Homeostasis and Metabolic Integrity of Alveolar Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Seung Geun Song,Ein Lee,Jaemoon Koh,Doo Hyun Chung

STAR protocols 4:102053 PubMed36853720

2023

In vitro expansion of Wilms' tumor protein 1 epitope-specific primary T cells from healthy human peripheral blood mononuclear cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sanne van der Heijden,Donovan Flumens,Maarten Versteven,Stefanie Peeters,Hans De Reu,Diana Campillo-Davo,Yannick Willemen,Benson Ogunjimi,Viggo Van Tendeloo,Zwi N Berneman,Sébastien Anguille,Evelien Smits,Eva Lion

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-SDHA 抗体 [2E3GC12FB2AE2]

Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473)

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (AB154473)

関連する標識済み抗体及び組成の異なる製品 (3)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise