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AB217985

Alexa Fluor® 488 Anti-SDF1 抗体 [EPR1216]

Alexa Fluor® 488 Anti-SDF1 antibody [EPR1216]

1

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal SDF1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

別名を表示する

SDF1, SDF1A, SDF1B, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDF1 antibody [EPR1216] (AB217985)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDF1 antibody [EPR1216] (AB217985)

ab217985 staining SDF1 in THP-1 cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab217985 at 1/1000 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

関連する標識済み抗体及び組成の異なる製品 (5)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR1216

アイソタイプ

IgG

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

No

交差種

Human, Human

アプリケーション

ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in THP-1 cells fixed with 4% formaldehyde (10 min)</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The stromal cell-derived factor 1 (SDF1) also known as C-X-C motif chemokine 12 (CXCL12) is a chemokine protein that is important in immunological responses and cellular signaling. This protein weighs approximately 8 kDa. SDF1 is largely expressed in bone marrow stroma liver and endothelium of various tissues positioning it as an integral player in cell migration and homing processes. The protein functions as a chemoattractant for lymphocytes promoting cellular trafficking and organ development.
Biological function summary

SDF1 influences the migration and survival of hematopoietic progenitor cells. It plays a pivotal role in heart development angiogenesis and neuronal protein regulation. SDF1 binds with high affinity to its receptor CXCR4 forming a critical signal transduction complex that modulates cellular movement and growth responses. This interaction is important in the regulation of cell positioning and potential pathways of pathological changes.

Pathways

SDF1 has an important role in the chemokine signaling pathway and is involved in the pathways controlling hematopoietic stem cell migration and homing. The interaction between SDF1 and CXCR4 triggers downstream signaling events engaging proteins like PI3K and MAPK which promote cell survival and proliferation. Furthermore the SDF1/CXCR4 axis is central to the vascular endothelial growth factor (VEGF) pathway facilitating angiogenesis and tissue repair mechanisms.

SDF1 relates closely to cancer metastasis and HIV infection. The SDF1/CXCR4 interaction acts as a co-receptor for HIV entry into host cells implicating it in viral pathogenesis. Overexpression of SDF1 and its binding partner CXCR4 contributes to tumor growth invasion and metastasis in various cancers by promoting angiogenesis and tumor cell migration. The targeting of the SDF1/CXCR4 axis holds therapeutic potential in cancer treatment and infectious disease management.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils (PubMed : 18802065, PubMed : 39093700). Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis (PubMed : 8752281, PubMed : 18802065, PubMed : 39093700). Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for CXCL12/SDF-1 (PubMed : 16107333, PubMed : 19255243). Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV : ITGB3, ITGA4 : ITGB1 and ITGA5 : ITGB1 in a CXCR4-independent manner (PubMed : 29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase (PubMed : 18802065). Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins (PubMed : 16107333, PubMed : 18802065, PubMed : 19255243, PubMed : 39093700). CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase (PubMed : 18802065). Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1 (PubMed : 8752281). Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation (By similarity). Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).. SDF-1-beta(3-72). Shows a reduced chemotactic activity.. SDF-1-alpha(3-67). Shows a reduced chemotactic activity (PubMed : 14525775). Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites (PubMed : 14525775).
See full target information CXCL12
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 12: PubMed36670936

2022

Specificity Protein 1-Mediated Promotion of CXCL12 Advances Endothelial Cell Metabolism and Proliferation in Pulmonary Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Evan R DeVallance,Christopher M Dustin,Daniel Simoes de Jesus,Imad Al Ghouleh,John C Sembrat,Eugenia Cifuentes-Pagano,Patrick J Pagano

International journal of molecular sciences 22: PubMed34884454

2021

Characterization of the Transient Deficiency of PKC Isozyme Levels in Immature Cord Blood T Cells and Its Connection to Anti-Allergic Cytokine Profiles of the Matured Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Khalida Perveen,Alex Quach,Michael J Stark,Susan L Prescott,Simon C Barry,Charles S Hii,Antonio Ferrante
View all publications
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代替製品

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AB84277

Biotin Anti-SDF1 antibody

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Primary Antibodies

AB9797

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Primary Antibodies

AB25117

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組合せ製品

Assay Kits

AB314389

Human SDF1 beta ELISA Kit

Sandwich ELISA - Human SDF1 beta ELISA Kit (AB314389)

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Primary Antibodies

AB208128

Alexa Fluor® 488 Anti-CXCR4 antibody [UMB2]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CXCR4 antibody [UMB2] (AB208128)

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Proteins & Peptides

AB259416

Recombinant human SDF1 protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human SDF1 protein (Active) (AB259416)

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Primary Antibodies

AB189524

Anti-CD44 antibody [EPR18668]

RabMAb|Recombinant|Lab Essentials|20ul selling size|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR18668] (AB189524)

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Assay Kits

AB100741

Mouse SDF1 ELISA Kit

ELISA - Mouse SDF1 ELISA Kit (AB100741)

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Abcam product promise

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