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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Alexa Fluor® 488 Anti-RBBP7 抗体 [EPR23796-74] (ab290984)

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Immunocytochemistry - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984)
  • Immunocytochemistry - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984)
  • Western blot - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984)
  • Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR23796-74] to RBBP7
  • Suitable for: WB, ICC
  • Knockout validated
  • Reacts with: Mouse, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 750 Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74]
    RBBP7 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 488 Rabbit monoclonal [EPR23796-74] to RBBP7

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • アプリケーション

    適用あり: WB, ICCmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse spleen tissue lysate; NIH/3T3 (mouse embryonic fibroblast) whole cell lysate. ICC: NIH/3T3 (mouse embryonic fibroblasts); wild-type HAP1 cell line.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290984の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500. Predicted molecular weight: 48 kDa.
ICC
1/50.
特記事項
WB
1/500. Predicted molecular weight: 48 kDa.
ICC
1/50.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.

  • 配列類似性

    Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
    Contains 7 WD repeats.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • G1/S transition control protein binding protein RbAp46 antibody
      • Histone acetyltransferase type B subunit 2 antibody
      • Histone binding protein RBBP7 antibody
      • Histone-binding protein RBBP7 antibody
      • MGC138867 antibody
      • MGC138868 antibody
      • Nucleosome remodeling factor subunit RBAP46 antibody
      • Nucleosome-remodeling factor subunit RBAP46 antibody
      • RBAP46 antibody
      • RBBP 7 antibody
      • RBBP-7 antibody
      • RBBP7 antibody
      • RBBP7_HUMAN antibody
      • Retinoblastoma binding protein 7 antibody
      • Retinoblastoma binding protein p46 antibody
      • Retinoblastoma-binding protein 7 antibody
      • Retinoblastoma-binding protein p46 antibody
      • Retinoblastoma-binding protein RbAp46 antibody
      see all

    画像

    • Immunocytochemistry - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984)
      Immunocytochemistry - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984)

      Immunofluorescent analysis of 4% Paraformaldehyde fixed RBBP7 knockout human chronic myelogenous leukemia near-haploid cell line permeabilized with 0.1% TritonX-100 labelling RBBP7 with ab290984 at 1/50 dilution, (Green). Confocal image showing nuclear staining in Wild-type HAP1 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (shown in Red) was used to counterstain tubulin at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

      Immunocytochemistry image showing no staining in RBBP7 knockout HAP1 cell line, and nuclear staining in wild type HAP1 cell line, which is specific.

    • Immunocytochemistry - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984)
      Immunocytochemistry - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984)

      Immunofluorescent analysis of 4% Paraformaldehyde fixed NIH/3T3 (mouse embryonic fibroblasts) permeabilized with 0.1% TritonX-100 labelling RBBP7 with ab290984 at 1/50 dilution, (Green). Confocal image showing nuclear staining in NIH/3T3 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (shown in Red) was used to counterstain tubulin at 1/200 dilution. The nuclear counterstain was DAPI (Blue). 

    • Western blot - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984)
      Western blot - Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984)
      All lanes : Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984) at 1/500 dilution

      Lane 1 : Mouse spleen tissue lysate
      Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
      Lane 3 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 48 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 7 seconds


      Blocking / diluent buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

      False colour image of Western blot: Anti-RBBP7 antibody (ab290984) staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody 6C5 loading control staining at 1/20000 dilution, shown in red.

    • Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984)
      Alexa Fluor® 488 Anti-RBBP7 antibody [EPR23796-74] (ab290984)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab290984 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290984 は論文での使用が確認できていません。

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