AB199091

Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb
Recombinant
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(33 Publications)

Anti-Rabbit IgG, monoclonal [EPR25A] - Isotype Control- Alexa Fluor® 488 conjugated (ab199091) is a rabbit recombinant monoclonal antibody used in Flow Cytometry, ICC/IF.

- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

41 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199091 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (pseudocolored in red). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow Cyt

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Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling TREM1 with ab316941 at 1/50 dilution (1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Human PBMC are co-stained with CD3 conjugated Alexa Fluor®647.
Gated on viable cell.

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow Cyt

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Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized EZH2 knockout HAP1 (Left) / parental HAP1 (HOWT01) (human chronic myelogenous leukemia near-haploid cell, Right) cells labelling KMT6 / EZH2 with ab317359 at 1/50 dilution(1ug)/(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Untreated HUVEC (human umbilical vein endothelial cell, Green) / HUVEC treated with 0.5ug/ml LPS for 24h and 300ng/ml BFA for 20h (Red) cells labelling IL-6 with ab314590 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human PBMC(human peripheral blood mononuclear cell) cells labelling T-bet / Tbx21 with ab315424 at 1/500 dilution (0.1ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control.

Human PBMC are co-stained with CD56 conjugated to BV421.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow Cyt

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Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast, Left) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling Fas with ab314884 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Negative control : K-562. Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling Fas with ab314884 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Gated on viable cells. Cells are co-stained with CD45RO conjugated to BV421.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with myc-tagged IL-23R expression vector containing a Myc tag (Middle) / 293T cells transfected with an empty vector containing a Myc tag (Right) cells labelling IL-23R with ab319083 at 1/5000 dilution (0.01ug)compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

Live cells were surface stained with ab319083, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 647.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / HEK-293T (human embryonic kidney epithelial cell) transfected with myc-tagged IL-22 expression vector containing a myc tag (Right) cells labelling IL-22 with ab317658 at 1/500 dilution (0.1ug) / Right (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow Cyt

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Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labelling CD300a with ab325226 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells.
Negative control : 293T (PMID : 22043923)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a mouse CD27 expression vector (Middle) / 293T cells transfected with an empty expression vector (Right) cells labelling CD27 with ab324176 at 1/5000 dilution (0.01ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control.

Cells were co-stained with Myc tag conjugated to Alexa Fluor®647.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Mutant p53 with ab305114 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC12A with ab317062 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control.

Gated on viable cells. Cells were co-stained with anti-CD11b conjugated to BV421.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / HeLa (human cervical adenocarcinoma epithelial cell, Right) cells labelling Mesothelin with ab315357 at 1/50 dilution (1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : MCF7 (PMID : 18245228). Gated on viable cell.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab198978 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab198978, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor^® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype / 293T (human embryonic kidney epithelial cell) transfected with a TRDC (human TCR delta) protein expression vector containing a myc-His tag(Up Right) / 293T transfected with a TRGC1 protein (human TCR gamma) expression vector (Down Left) / 293T transfected with an empty vector containing a myc-His tag (Down Right) cells labelling TCR delta with ab317064 at 1/500 dilution (0.1ug)/ Up Right, Down Left and Down Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometry overlay histogram showing left HeLa positive cells and right negative HeLa VCL KO stained with ab196454 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab196454) (1x 10⁶ in 100μl at 0.008μg/ml (1/62500)) for 30min at 22°C.

Isotype control antibody (black line) was Recombinant Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized human PBMC (human peripheral blood mononuclear cell) treated with 20ng/ml IFN-gamma for 24h cells labelling Sialoadhesin/CD169 with ab308426 at 1/50 dilution (1ug)/ (Right) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Left) Cells were co-stained with anti-CD11b conjugated to BV421.

Flow Cyt

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Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Survivin with ab307852 at 1/50 dilution (1.0ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) cells labelling TLR8 with ab324390 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control.

Cells were stained with anti-CD14 conjugated to BV421. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or our our antibody.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling TCR delta with ab317064 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control.

Gated on viable cells. Cells were co-stained with anti-CD4 conjugated to Alexa Fluor® 647.

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of Isotype (Left) / HEK-293T (human embryonic kidney epithelial cell) transfected with Myc-tagged ENPP3 overexpression construct (Middle) / 293T transfected with an empty vector containing a myc-His tag (Right) cells labelling ENPP3/B10 with ab319125 at 1/50 dilution (1ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

Cells were co-stained with anti-myc conjugated to Alexa Fluor 647.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Human PBMC are co-stained with CD11b conjugated BV421.
Gated on viable cell.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD11a with ab317289 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control.

Gated on viable cells. Cells are co-stained with CD3 conjugated to Alexa Fluor® 647.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of Human PBMC(human peripheral blood mononuclear cell) cells labelling CD45 with ab315958 at 1/5000 dilution (0.01ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control.

Human PBMCs are co-stained with CD3 conjugated Alexa Fluor®647.Gated on viable cell.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with ab325226 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control.

Cells were co-stained with anti human CD14 conjugated to Brilliant Violet 421.
Gated on viable cells.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labelling KMT6 / EZH2 with ab317359 at 1/50 dilution(1ug)/(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Cells were co-stained with anti human CD19 conjugated to PE/CY7.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T cells transfected with a mouse CCR4 expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling CCR4 with ab324335 at 1/5000 dilution (0.01ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Cells were surface stained with isotype control or our antibody. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Human PBMC are co-stained with CD19 conjugated to Alexa Fluor®647, showing a distinct CD19 positive population with no cross reactivity to TBX21 positive cells.

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293 (human embryonic kidney epithelial cell) cells labelling Mutant p53 with ab305114 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR25A

アイソタイプ

IgG

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

アプリケーション

Flow Cyt, Flow Cyt (Intra), ICC/IF

applications

特異性

Please note Abcam have optimised the validation of this product. In our hands, we observe an increase in background signal intensity with the use of Triton X-100 and would recommend using an alternative permeabilisation method such as methanol or saponin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.", "FlowCytIntra-species-checked": "", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

Product Specifications
Alexa Fluor^® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, Flow Cyt (Intra) and ICC/IF.

Quality and Validation
Alexa Fluor^® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091) has been cited over 25 times in peer reviewed journals and is trusted by the scientific community.
Flourescent conjugated antibodies are ideal tools for multiplex IHC and flow cytometry experiments.
Alexa Fluor^® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091) has 25 independent reviews from customers.
Alexa Fluor^® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091) specifically detects rabbit IgG and is sold in 100 µL selling sizes.

Related Products
Conjugation-ready, carrier free format available for antibody clone (EPR25A) - ab210849.

Immunogen
Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

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文献 (33)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40100289

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AMPK is necessary for Treg functional adaptation to microenvironmental stress during malignancy and viral pneumonia.

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Manuel A Torres Acosta,Jonathan K Gurkan,Qianli Liu,Nurbek Mambetsariev,Carla Reyes Flores,Kathryn A Helmin,Anthony M Joudi,Luisa Morales-Nebreda,Kathleen Cheng,Hiam Abdala-Valencia,Samuel E Weinberg,Benjamin D Singer

Heliyon 10:e40734 PubMed39717606

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Serum ADAM17 levels pre-antiviral therapy correlate with HIV patient immune reconstitution.

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Ying Lin,Ming Chen,Xuelian Zhu, Xiaoya Cui,Suyu Sun,Xingzhong Hu

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Xingying Zhang,Chenze Zhang,Shan Lu,Jingxi Dong,Na Tang,Yao Wang,Weidong Han,Xi Pan,Xiang Zhang,Duan Liu,Ng Shyh-Chang,Yu Wang,Guihai Feng,Haoyi Wang

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IRF1 governs the expression of SMARCC1 via the GCN5-SETD2 axis and actively engages in the advancement of osteoarthritis.

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Dong Wang,Yujun Zhang,Liangping Zhang,Du He,Lan Zhao,Zhimin Miao,Wei Cheng,Chengyue Zhu,Li Zhu,Wei Zhang,Hongting Jin,Hang Zhu,Hao Pan

Journal of translational medicine 22:5 PubMed38169393

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PIGT promotes cell growth, glycolysis, and metastasis in bladder cancer by modulating GLUT1 glycosylation and membrane trafficking.

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Mingyue Tan,Qi Pan,Chao Yu,Xinyu Zhai,Jianyi Gu,Le Tao,Dongliang Xu

Journal of experimental & clinical cancer research : CR 42:304 PubMed37974213

2023

Targeting FAcilitates Chromatin Transcription complex inhibits pleural mesothelioma and enhances immunotherapy.

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Anand Singh,Nathanael Pruett,Shivani Dixit,Sudheer K Gara,Haitao Wang,Roma Pahwa,David S Schrump,Chuong D Hoang

Cellular & molecular biology letters 28:91 PubMed37946128

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Irf7 regulates the expression of Srg3 and ferroptosis axis aggravated sepsis-induced acute lung injury.

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Xinyu Ling,Shiyou Wei,Dandan Ling,Siqi Cao,Rui Chang,Qiuyun Wang,Zhize Yuan

Small (Weinheim an der Bergstrasse, Germany) 19:e2300578 PubMed37423970

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An oncolytic poxvirus encoding hNIS, shows anti-tumor efficacy and allows tumor imaging in a liver cancer model.

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Engineering of a Bispecific Nanofitin with Immune Checkpoint Inhibitory Activity Conditioned by the Cross-Arm Binding to EGFR and PDL1.

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Perrine Jacquot,Javier Muñoz-Garcia,Maurine Fleury,Denis Cochonneau,Rémi Gaussin,Elise Enouf,Caroline Roze,Emilie Ollivier,Mathieu Cinier,Dominique Heymann

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Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control