Alexa Fluor® 488 Anti-PGP9.5 抗体 [EPR4118] - Neuronal Marker (ab302578)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab302578)](https://www.abcam.co.jp/ps/products/302/ab302578/Images/AB302578-488703-anti-pgp95-antibody-epr4118-icc-sh-sy5y.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab302578)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR4118] to PGP9.5 - Neuronal Marker
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 488 Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker
PGP9.5 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 488 Rabbit monoclonal [EPR4118] to PGP9.5 - Neuronal Marker -
由来種
Rabbit -
標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
アプリケーション
適用あり: ICC/IFmore details
適用なし: IHC-Fr -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC/IF: SH-SY5Y and Neuro-2a cell lines.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4118 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 647 Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab196173)
- Anti-PGP9.5 antibody [EPR4118] - BSA and Azide free (ab220823)
- Alexa Fluor® 555 Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab300549)
- Alexa Fluor® 594 Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab302664)
- Alexa Fluor® 568 Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab305109)
- APC Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab317591)
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302578の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/50.
|
| 特記事項 |
|---|
|
ICC/IF
1/50. |
ターゲット情報
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機能
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. -
組織特異性
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. -
関連疾患
Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
配列類似性
Belongs to the peptidase C12 family. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain. - Information by UniProt
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参照データベース
- Entrez Gene: 7345 Human
- Entrez Gene: 22223 Mouse
- Omim: 191342 Human
- SwissProt: P09936 Human
- SwissProt: Q9R0P9 Mouse
- Unigene: 518731 Human
- Unigene: 29807 Mouse
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別名
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab302578)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cells) labeling PGP9.5 with ab302578 at 1/50 dilution (10.0 μg/ml) (green). Confocal image showing cytoplasmic and nuclear staining in SH-SY5Y cells. The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (2.5 μg/mL) (red).
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab302578)](https://www.abcam.co.jp/ps/products/302/ab302578/Images/AB302578-488702-anti-pgp95-antibody-epr4118-icc-neuro-2a.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab302578)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblasts) labeling PGP9.5 with ab302578 at 1/50 dilution (10.0 μg/ml) (green). Confocal image showing cytoplasmic and nuclear staining in Neuro-2a cells. The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (2.5 μg/mL) (red).
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![Alexa Fluor® 488 Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab302578)](https://www.abcam.co.jp/ps/products/302/ab302578/Images/AB302578-1-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 488 Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (ab302578)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab302578 は論文での使用が確認できていません。