AB302972

Alexa Fluor® 488 Anti-Pancreatic Polypeptide 抗体 [EPR23320-10]

Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Pancreatic Polypeptide antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

別名を表示する

PNP, PPY, Pancreatic polypeptide prohormone, PH, Pancreatic polypeptide Y

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB302972)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB302972)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue stained for Pancreatic Polypeptide using ab302972 at a 1/100 dilution (5.0 μg/ml) (Green). Anti-PPY stained on pancreatic islets shown in green; anti-PGP9.5 (ab196173) stained on pancreatic islets shown in red. The section was incubated with ab302972 and ab196173 at 4°C overnight (shown in green and red respectively). Nuclear DNA was labelled with DAPI (Blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB302972)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue stained for Pancreatic Polypeptide using ab302972 at a 1/100 dilution (5.0 μg/ml) (Green). Positive staining on mouse pancreatic islet. The section was incubated with ab302972 at 4°C overnight (Green). Nuclear DNA was labelled with DAPI (Blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB302972)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue stained for Pancreatic Polypeptide using ab302972 at a 1/100 dilution (5.0 μg/ml) (Green). Positive staining on rat pancreatic islet. The section was incubated with ab302972 at 4°C overnight (Green). Nuclear DNA was labelled with DAPI (Blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR23320-10

アイソタイプ

IgG

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

交差種

Human, Mouse, Rat, Human, Mouse, Rat

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Pancreatic Polypeptide also known as PP is a small protein with a molecular mass approximately 4200 Da. It is secreted by PP cells in the pancreas particularly in the F-cells located in the Langerhans islets. This peptide is highly conserved among different species including the rat where it displays similar function and expression patterns. Pancreatic polypeptide regulates various physiological processes by binding to specific receptors on target tissues.

Biological function summary

In various mammalian species including rats pancreatic polypeptide plays a significant role in the regulation of appetite and pancreatic secretion. It is not recognized as part of a multi-protein complex but it directly influences the function of digestive organs by inhibiting pancreatic exocrine secretions and gallbladder contraction. This makes it an important mediator in controlling the body's energy balance and digestive functions.

Pathways

Pancreatic polypeptide is involved in the peptidergic pathway regulating energy homeostasis and digestive processes. It interacts with the Y receptor family particularly the Y4 receptor which contributes to its regulatory actions on appetite and digestive enzyme secretion. This peptide works alongside other polypeptides such as neuropeptide Y and peptide YY in mediating these complex physiological pathways.

Pancreatic polypeptide levels relate to conditions such as obesity and diabetes. Altered secretion of this peptide can contribute to obesity by affecting appetite regulation and energy homeostasis. Furthermore in diabetes pancreatic polypeptide is connected to abnormal pancreatic function often observed alongside insulin and glucagon dysregulation. Understanding these relationships can provide insight into therapeutic strategies targeting metabolic and endocrine disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Pancreatic polypeptide. Hormone secreted by pancreatic cells that acts as a regulator of pancreatic and gastrointestinal functions probably by signaling through the G protein-coupled receptor NPY4R2.

See full target information PPY

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Pancreatic Polypeptide 抗体 [EPR23320-10]

Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB302972)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB302972)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB302972)

関連する標識済み抗体及び組成の異なる製品 (3)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise