AB277270

Alexa Fluor® 488 Anti-pan Cytokeratin 抗体 [C-11]

Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11]

Recombinant
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(6 Publications)

Anti-pan Cytokeratin antibody [C-11] - Alexa Fluor® 488 conjugated (ab277270) is a mouse recombinant monoclonal antibody detecting pan Cytokeratin in Flow Cytometry (Intra). Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1, 39.1, Cytokeratin-17, Keratin-17, CK-17, K17, KRT17, SCL, KRT7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7, CK7, CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18, Cytokeratin-19, Keratin-19, CK-19, K19, KRT19, ck19, ck 19, K6A, KRT6D, KRT6A, Cytokeratin-6A, Cytokeratin-6D, Keratin-6A, Type-II keratin Kb6, CK-6A, CK-6D, KPP, KRT10, Cytokeratin-10, Keratin-10, CK-10, K10, KRT2A, KRT2E, KRT2, Cytokeratin-2e, Epithelial keratin-2e, Keratin-2 epidermis, Keratin-2e, Type-II keratin Kb2, CK-2e, K2e, CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8, Cytokeratin-14, Keratin-14, CK-14, K14, KRT14, KRTB, KRT15, Cytokeratin-15, Keratin-15, CK-15, K15, 58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK-5, K5, KRT5, CK5, CYK4, KRT4, Cytokeratin-4, Keratin-4, Type-II keratin Kb4, CK-4, K4, Cytokeratin-20, Keratin-20, Protein IT, CK-20, K20, KRT20, KRT16A, KRT16, Cytokeratin-16, Keratin-16, CK-16, K16, KRT6E, KRT6C, Cytokeratin-6C, Cytokeratin-6E, Keratin K6h, Keratin-6C, Type-II keratin Kb12, CK-6C, CK-6E, K6C, K6B, KRTL1, KRT6B, Cytokeratin-6B, Keratin-6B, Type-II keratin Kb10, CK-6B, Cytokeratin-13, Keratin-13, CK-13, K13, KRT13, Cytokeratin-12, Keratin-12, CK-12, K12, KRT12, 65 kDa cytokeratin, Cytokeratin-3, Keratin-3, Type-II keratin Kb3, CK-3, K3, KRT3, Cytokeratin-9, Keratin-9, CK-9, K9, KRT9

2 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] (AB277270)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] (AB277270)

Flow cytometry overlay histogram showing left HeLa positive cells and right negative Jurkat cells stained with ab277270 (red line). The cells were fixed with 80 % methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab277270) (1x 10⁶ in 100μL at 0.2μg/mL (1/2500)) for 30 min at 22°C.

Isotype control antibody (black line) was Mouse IgG1κ Alexa Fluor^® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

This antibody gave a positive signal in HeLa cells fixed with 4 % formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Lab

Immunocytochemistry - Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] (AB277270)

ab277270 staining pan Cytokeratin cells. The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab277270 at at 1µg/ml (shown in green) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor^® 647), pre-adsorbed at 1/250 dilution (shown in magenta) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 minutes).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

C-11

アイソタイプ

IgG1

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

交差種

Human, Human, Mouse, Rat

アプリケーション

IHC-P, ICC, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

What is this antibody validated in?
Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] (ab277270) is a mouse recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone C-11 is also available pre-conjugated to a variety of labels for your convenience – Anti-pan Cytokeratin Alexa Fluor® 488 [C-11] (ab277270).

Other related products
We have a range of other formats of antibody clone [C-11] also available for your convenience: ab7753, PE - ab52460, APC - ab106166, Carrier free - ab264485, ab269366, Alexa Fluor® 594 - ab277234, Alexa Fluor® 488 - ab277270, Alexa Fluor® 555 - ab279324, Oligonucleotide - ab279360, Alexa Fluor® 647 - ab309978

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.

Biological function summary

Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.

Pathways

Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).

Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

See full target information KRT1

追加のターゲット

KRT7,KRT4,KRT19,Keratin, type I cytoskeletal 9,KRT17,KRT3,Keratin, type I cytoskeletal 14,KRT6C,KRT6B,KRT20,KRT18,Keratin, type II cytoskeletal 5,KRT8,KRT16,KRT10,KRT15,Keratin, type I cytoskeletal 13,KRT12,KRT6A,KRT2

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Lab on a chip 25:5292-5301 PubMed40878245

2025

Benchmarking microfluidic and immunomagnetic platforms for isolating circulating tumor cells in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Celine Macaraniag,Ifra Khan,Alexandra Barabanova,Valentina Valle,Jian Zhou,Pier C Giulianotti,Alain Borgeat,Gina Votta-Velis,Ian Papautsky

Biology open 12: PubMed37194999

2023

Bioengineering of a tumour-stroma 3D-tumouroid co-culture model of hypopharyngeal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Santu Saha,Rachel Howarth,Sweta Sharma-Saha,Charles Kelly

International journal of molecular sciences 24: PubMed37108635

2023

Hydrolyzed Conchiolin Protein (HCP) Extracted from Pearls Antagonizes both ET-1 and -MSH for Skin Whitening.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Yang,Zhekun Wang,Yunwei Hu,Kaile Zong,Xingjiang Zhang,Hui Ke,Pan Wang,Yuyo Go,Xi Hui Felicia Chan,Jianxin Wu,Qing Huang

Science advances 8:eabo7792 PubMed36054348

2022

Genome-wide in vivo screen of circulating tumor cells identifies as a regulator of metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Xia,Yuan Ma,Kangfu Chen,Bill Duong,Sharif Ahmed,Randy Atwal,David Philpott,Troy Ketela,Jennifer Pantea,Sichun Lin,Stephane Angers,Shana O Kelley

Reproduction & fertility 3:30-38 PubMed35350653

2022

Altered endometrial oestrogen-responsiveness and recurrent reproductive failure.

Applications

Unspecified application

Species

Unspecified reactive species

Hannan Al-Lamee,Amy Ellison,Josephine Drury,Christopher J Hill,Andrew J Drakeley,Dharani K Hapangama,Nicola Tempest

Journal of neurophysiology 106:309-18 PubMed21525372

2011

Reduced expression of SynGAP, a neuronal GTPase-activating protein, enhances capsaicin-induced peripheral sensitization.

Applications

Unspecified application

Species

Unspecified reactive species

Djane Braz Duarte,Jian-Hong Duan,Grant D Nicol,Michael R Vasko,Cynthia M Hingtgen

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-pan Cytokeratin 抗体 [C-11]

Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11]

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] (AB277270)

Immunocytochemistry - Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] (AB277270)

関連する標識済み抗体及び組成の異なる製品 (6)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (6)

Abcam product promise