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AB314281

Alexa Fluor® 488 Anti-Myeloperoxidase 抗体 [SP72]

Alexa Fluor® 488 Anti-Myeloperoxidase antibody [SP72]

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Rabbit Recombinant Monoclonal Myeloperoxidase antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples.

別名を表示する

Myeloperoxidase, MPO

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Myeloperoxidase antibody [SP72] (AB314281)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Myeloperoxidase antibody [SP72] (AB314281)

Negative immunofluorescence staining of Myeloperoxidase in a section of formalin-fixed paraffin-embedded human normal cerebellum. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab314281 at 1/500 dilution. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Myeloperoxidase antibody [SP72] (AB314281)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Myeloperoxidase antibody [SP72] (AB314281)

Immunofluorescence staining of Myeloperoxidase in a section of formalin-fixed paraffin-embedded normal human tonsil *. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab314281 at 1/500 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

関連する標識済み抗体及び組成の異なる製品 (10)

  • Unconjugated

    Anti-Myeloperoxidase antibody [SP72]

  • Carrier free

    Anti-Myeloperoxidase antibody [SP72] - BSA and Azide free

  • 660 APC

    APC Anti-Myeloperoxidase antibody [SP72]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Myeloperoxidase antibody [SP72]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Myeloperoxidase antibody [SP72]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Myeloperoxidase antibody [SP72]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Myeloperoxidase antibody [SP72]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Myeloperoxidase antibody [SP72]

  • HRP

    HRP Anti-Myeloperoxidase antibody [SP72]

  • 578 PE

    PE Anti-Myeloperoxidase antibody [SP72]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP72

アイソタイプ

IgG

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

No

交差種

Human, Human

アプリケーション

Flow Cyt (Intra), IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB105136

Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric)

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (AB105136)

  • 4
  • 3
Primary Antibodies

AB5103

Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (AB5103)

  • 4
  • 28
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB275587

FITC Anti-CD66b antibody [04]

Flow Cytometry - FITC Anti-CD66b antibody [04] (AB275587)

  • 0
  • 0
Primary Antibodies

AB199093

Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).
See full target information MPO
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Abcam product promise

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