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AB275189

Alexa Fluor® 488 Anti-L1CAM 抗体 [EPR23241-224]

Alexa Fluor® 488 Anti-L1CAM antibody [EPR23241-224]

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Rabbit Recombinant Monoclonal L1CAM antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt, IHC-P and reacts with Human samples.

別名を表示する

CD171, CAML1, MIC5, L1CAM, Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1

2 Images
Flow Cytometry - Alexa Fluor® 488 Anti-L1CAM antibody [EPR23241-224] (AB275189)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-L1CAM antibody [EPR23241-224] (AB275189)

Flow cytometry overlay histogram showing right HeLa positive cells and left negative L1CAM knockout HeLa cells (ab255401) cells stained with ab275189 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab275189) (1x 106 in 100μl at 10μg/ml (1/50)) for 30 min on ice. Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor 488® (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. 5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-L1CAM antibody [EPR23241-224] (AB275189)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-L1CAM antibody [EPR23241-224] (AB275189)

ab275189 staining L1CAM in wild-type HeLa cells, with negative expression in L1CAM knockout HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab275189 at 1 μg/ml (shown in Green). ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647) (shown in red) was used at 2 μg/ml for structural counterstaining.

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

This product also work with 4% formaldehyde (10 min) fixation under the same testing conditions.

関連する標識済み抗体及び組成の異なる製品 (7)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR23241-224

アイソタイプ

IgG

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

No

交差種

Human, Human

アプリケーション

IHC-P, ICC/IF, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

L1CAM also known as neural cell adhesion molecule L1 is a transmembrane protein with a molecular weight of approximately 200-220 kDa. The L1CAM protein belongs to the immunoglobulin superfamily and is heavily glycosylated. It plays a significant role in cell adhesion processes within the nervous system. L1CAM is expressed in a variety of tissues including the brain kidney and peripheral nerves and is notably present in neuronal cells where it engages in cell-cell interactions. Researchers frequently use L1CAM IHC ELISA methods and specific fluorescent markers like Alexa Fluor 568 to study its expression patterns and localization.
Biological function summary

L1CAM facilitates neuronal migration axonal growth and synaptic plasticity. This protein participates in the formation and maintenance of neural networks. It interacts with other cell adhesion molecules and components of the extracellular matrix. L1CAM acts as an important modulator within neuronal signaling complexes which impacts the development and regeneration of the nervous system. Insights into its structural and cell interaction properties have made it a focus for understanding nervous system functions.

Pathways

L1CAM participates extensively in the MAPK and PI3K/AKT pathways. These pathways contribute to cellular growth survival and programmed cell death. The protein interacts with other molecules such as integrins and FGFRs to propagate signaling cascades important for cellular response mechanisms. These connections help modulate cytoskeletal dynamics influencing cellular adhesion and motility needed during brain development and response to injury.

L1CAM has been associated with hydrocephalus and some cancers. Mutations in the L1CAM gene can lead to L1 syndrome which includes a spectrum of neurological disorders particularly hydrocephalus. In cancer overexpression or altered regulation of L1CAM may correlate with tumor aggressiveness and metastatic potential. L1CAM's association with integrin and cadherin proteins underlines its relevance in pathological states and highlights a potential target for therapeutic interventions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
See full target information L1CAM
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組合せ製品

Assay Kits

AB278114

Human L1CAM ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

  • 0
  • 0
Primary Antibodies

AB199093

Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

  • 0
  • 0
Primary Antibodies

AB314283

Alexa Fluor® 488 Anti-Tau antibody [SP70]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Tau antibody [SP70] (AB314283)

  • 0
  • 0
Primary Antibodies

AB242199

Anti-MACC1 antibody [CL0856]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

  • 0
  • 0
Primary Antibodies

AB201679

Alexa Fluor® 647 Anti-CNPase antibody [11-5B]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CNPase antibody [11-5B] (AB201679)

  • 0
  • 0

Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com