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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Alexa Fluor® 488 Anti-KDM1/LSD1 抗体 [EPR6825] - Nuclear Marker (ab184811)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab184811)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab184811)
  • Alexa Fluor® 488 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab184811)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR6825] to KDM1/LSD1 - Nuclear Marker
  • Suitable for: ICC/IF
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free HRP PE Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 488 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker
    KDM1/LSD1 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 488 Rabbit monoclonal [EPR6825] to KDM1/LSD1 - Nuclear Marker

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • アプリケーション

    適用あり: ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: HeLa cells, HAP1-KDM1A cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR6825

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184811の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/50 - 1/100.
特記事項
ICC/IF
1/50 - 1/100.

ターゲット情報

  • 機能

    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.

  • 組織特異性

    Ubiquitously expressed.

  • 配列類似性

    Belongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.

  • ドメイン

    The SWIRM domain may act as an anchor site for a histone tail.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Amine oxidase (flavin containing) domain 2 antibody
      • Amine oxidase, flavin containing, 2 antibody
      • AOF2 antibody
      • BHC110 antibody
      • BRAF35 HDAC complex protein BHC110 antibody
      • BRAF35-HDAC complex protein BHC110 antibody
      • BRAF35/HDAC complex, 110-kD subunit antibody
      • CPRF antibody
      • EC1 antibody
      • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
      • Flavin-containing amine oxidase domain-containing protein 2 antibody
      • KDM 1 antibody
      • KDM1 antibody
      • Kdm1a antibody
      • KDM1A_HUMAN antibody
      • KIAA0601 antibody
      • LSD 1 antibody
      • LSD1 antibody
      • Lysine (K) specific demethylase 1 antibody
      • Lysine (K) specific demethylase 1A antibody
      • Lysine demethylase 1A antibody
      • Lysine specific histone demethylase 1 antibody
      • Lysine specific histone demethylase 1A antibody
      • Lysine-specific demethylase 1 antibody
      • Lysine-specific demethylase 1A antibody
      • Lysine-specific histone demethylase 1A antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab184811)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab184811)

      ab184811 staining KDM1A in wild-type HAP1 cells (top panel) and KDM1A knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab184811 at 1/500 dilution (shown in green) and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab184811)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab184811)

      ab184811 staining KDM1/ LSD1 in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab184811 at a working dilution of 1 in 100 overnight at +4°C (shown in green). AlexaFluor®350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410).

    • Alexa Fluor® 488 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab184811)
      Alexa Fluor® 488 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab184811)

    参考文献 (0)

    ab184811 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184811 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Flow Cytometry abreview for Anti-KDM1 / LSD1 antibody [EPR6825] - Nuclear Marker (Alexa Fluor® 488)

     Inconclusive
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (splenic B cells)
    Permeabilization
    Yes - Saponin-based permeabilization through the BD fixation/permeabilization solution kit with BD GolgiPlug (555028)
    Gating Strategy
    Gated out doublets and dump negative cells (CD11b-, Thy1.2-, F4/80-), then gated on all B220+ cells (B cells) and CD138+ cells (plasma cells).
    Specification
    splenic B cells
    Preparation
    Cell harvesting/tissue preparation method: Sacked mice in CO2 chamber for 10 minutes. Dissected out spleen. Manually crushed spleen through 40 micrometer cell strainer. Lysed RBCs with ACK lysis buffer. Resuspended in FACS buffer, stained, flowed.
    Sample buffer: FACS buffer: 1X PBS, 2 mM EDTA, 10 g BSA/liter
    Fixation
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Robert Haines

    Verified customer

    投稿 Jun 20 2017

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