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リコンビナント

Alexa Fluor® 488 Anti-Hsp60 抗体 [EPR18245-93] (ab284641)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)
  • Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR18245-93] to Hsp60
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93]
    Hsp60 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 488 Rabbit monoclonal [EPR18245-93] to Hsp60

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • アプリケーション

    適用あり: ICC/IF, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: HeLa (human cervix adenocarcinoma epithelial cells); NIH/3T3 (mouse embryonic fibroblasts) Flow Cyt (Intra): HeLa (Human cervix adenocarcinoma epithelial cells); NIH/3T3 (Mouse embryonic fibroblasts)

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab284641の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/500.
Flow Cyt (Intra)
1/500.
特記事項
ICC/IF
1/500.
Flow Cyt (Intra)
1/500.

ターゲット情報

  • 機能

    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

  • 関連疾患

    Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.

  • 配列類似性

    Belongs to the chaperonin (HSP60) family.

  • 細胞内局在

    Mitochondrion matrix.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 60 kDa chaperonin antibody
      • 60 kDa heat shock protein, mitochondrial antibody
      • CH60_HUMAN antibody
      • Chaperonin 60 antibody
      • Chaperonin, 60-KD antibody
      • CPN60 antibody
      • fa04a05 antibody
      • GROEL antibody
      • heat shock 60kDa protein 1 (chaperonin) antibody
      • Heat shock protein 1 (chaperonin) antibody
      • Heat shock protein 60 antibody
      • Heat shock protein 65 antibody
      • heat shock protein family D (Hsp60) member 1 antibody
      • HLD4 antibody
      • Hsp 60 antibody
      • HSP 65 antibody
      • HSP-60 antibody
      • HSP60 antibody
      • HSP65 antibody
      • HSPD1 antibody
      • HuCHA60 antibody
      • Mitochondrial matrix protein P1 antibody
      • P60 lymphocyte protein antibody
      • short heat shock protein 60 Hsp60s1 antibody
      • SPG13 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)

      Immunofluorescent analysis of 4% Paraformaldehyde fixed HeLa (human cervix adenocarcinoma epithelial cells) permeabilized with 0.1% TritonX-100 labelling Hsp60 with ab284641 at 1/500 dilution, (Green). Confocal image showing cytoplasmic staining in HeLa cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (shown in Red) was used to counterstain tubulin at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)

      Immunofluorescent analysis of 4% Paraformaldehyde fixed NIH/3T3 (mouse embryonic fibroblasts) permeabilized with 0.1% TritonX-100 labelling Hsp60 with ab284641 at 1/500 dilution, (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (shown in Red) was used to counterstain tubulin at 1/200 dilution. The nuclear counterstain was DAPI (Blue). 

    • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)
      Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)

      Flow cytometric overlay histogram of 4% paraformaldehyde fixed HeLa (Human cervix adenocarcinoma epithelial cells) permeabilized with 90% methanol labelling Hsp60 with ab284641 at 1/500 dilution (Red). Isotype control: Rabbit monoclonal isotype control - Alexa Fluor® 488 (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue). Gated on viable cells.

    • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)
      Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)

      Flow cytometric overlay histogram of 4% paraformaldehyde fixed NIH/3T3 (Mouse embryonic fibroblast) permeabilized with 90% methanol labelling Hsp60 with ab284641 at 1/500 dilution (Red). Isotype control: Rabbit monoclonal isotype control - Alexa Fluor® 488 (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue).

    • Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)
      Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] (ab284641)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab284641 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab284641 は論文での使用が確認できていません。

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