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リコンビナントRabMAb

Alexa Fluor® 488 Anti-Hexokinase 1 抗体 [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab184818)

Reviews (1) Submit a question References (1)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab184818)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab184818)
  • Alexa Fluor® 488 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab184818)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR10134(B)] to Hexokinase 1 - Mitochondrial Outer Membrane Marker
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 750 Carrier Free Unconjugated Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 488 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker
    Hexokinase 1 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 488 Rabbit monoclonal [EPR10134(B)] to Hexokinase 1 - Mitochondrial Outer Membrane Marker

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • アプリケーション

    適用あり: ICC/IF, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184818の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/50 - 1/100.
Flow Cyt (Intra)
1/50.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.
特記事項
ICC/IF
1/50 - 1/100.
Flow Cyt (Intra)
1/50.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 組織特異性

    Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.

  • パスウェイ

    Carbohydrate metabolism; hexose metabolism.

  • 関連疾患

    Hexokinase deficiency
    Neuropathy, hereditary motor and sensory, Russe type

  • 配列類似性

    Belongs to the hexokinase family.
    Contains 2 hexokinase domains.

  • ドメイン

    The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.

  • 細胞内局在

    Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • BB404130 antibody
    • Brain form hexokinase antibody
    • dea antibody
    • DrHXK1 antibody
    • EC 2.7.1.1 antibody
    • Glycolytic enzyme antibody
    • HEXOKIN antibody
    • hexokinase I antibody
    • Hexokinase PI antibody
    • Hexokinase type I antibody
    • Hexokinase, tumor isozyme antibody
    • Hexokinase-1 antibody
    • Hexokinase-A antibody
    • HK I antibody
    • HK1 antibody
    • HK1 tb antibody
    • HK1 tc antibody
    • Hk1-s antibody
    • HK1-ta antibody
    • HK1-tb antibody
    • HK1-tc antibody
    • HKD antibody
    • HKI antibody
    • HMSNR antibody
    • HXK1 antibody
    • HXK1_HUMAN antibody
    • im:7148527 antibody
    • mHk1-s antibody
    • wu:fc09d08 antibody
    • wu:fc16e02 antibody
    • wu:fc21e02 antibody
    • wu:fq14b11 antibody
    • zgc:55790 antibody
    • zgc:77618 antibody
    see all

画像

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab184818)
    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab184818)

    Overlay histogram showing HeLa cells stained with ab184818 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184818, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab184818)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab184818)

    ab184818 staining Hexokinase 1 in HeLa cells. The cells were fixed with 4% PFA (10min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab184818 at a working dilution of 1 in 100 overnight at +4°C (shown in green). AlexaFluor® 350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410).

  • Alexa Fluor® 488 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab184818)
    Alexa Fluor® 488 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab184818)

参考文献 (1)

ab184818 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184818 は 1 報の論文で使用されています。

  • Franchina DG  et al. Glutathione-dependent redox balance characterizes the distinct metabolic properties of follicular and marginal zone B cells. Nat Commun 13:1789 (2022). PubMed: 35379825

レビューと Q&A

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Flow Cytometry abreview for Alexa Fluor® 488 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (neonatal mouse intestines)
Permeabilization
Yes - commercial available pereabillization buffer
Gating Strategy
CD31+CD45-
Specification
neonatal mouse intestines
Fixation
commercial available IC fixation buffer
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Oct 19 2022

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