Alexa Fluor® 488 Anti-GFAP 抗体 [EPR1034Y]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB194324)

Immunofluorescence staining of GFAP staining in a section of formalin-fixed paraffin-embedded human Alzheimer’s brain*.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (Ph9.0) using retrieval settings of 100°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab194324 at 1/500 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB194324)

Image A : composite multiplex immunohistochemistry staining using conjugates panel composed of anti- GFAP (Alexa Fluor^® 488) (ab194324, 1/100 dilution, 5 µg/mL) (green), anti-MOG (Alexa Fluor^® 594) (ab305372, 1/100 dilution, 5 µg/mL) (magenta) and anti-Iba1 (Alexa Fluor^® 647) (ab225261, 1/100 dilution, 5 µg/mL) (white) on Formalin/PFA-fixed paraffin-embedded sections of rat cerebellum. Nuclear DNA was labelled with DAPI (blue).

Image B : Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti- GFAP (Alexa Fluor^® 488) (ab194324, 1/100 dilution, 5 µg/mL).

Image C : Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti- MOG (Alexa Fluor^® 594) (ab305372, 1/100 dilution, 5 µg/mL).

Image D : Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti- Iba1 (Alexa Fluor^® 647) (ab225261, 1/100 dilution, 5 µg/mL).

Immunohistochemistry staining was performed on a Leica Biosystems BOND^® RX instrument. Heat mediated antigen retrieval with EDTA (pH 9.0, 110°C) for 40 mins. The section was then incubated at room temperature for 1 hour and mounted using Dako Fluorescence Mounting Medium^®.

Image acquisition was performed with Leica SP8 confocal microscope. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB194324)

IHC-Fr image of GFAP staining in a section of frozen rat brain (hypothalamus). The section was air dried on the bench for 30 minutes and blocked for 2 hours in 1XPBS/0.1% Tween 20/1% BSA/0.3M Glycine buffer. The sample was then incubated overnight at +4°C with ab194324 at 1/50 dilution (shown in green). DAPI-containing mounting media was added to the sample (shown in blue). The inset negative control image is taken from an identical assay without primary antibody.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB194324)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GFAP with ab313596 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing positive staining in rat primary glia cells.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab194324 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB194324)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GFAP with ab313596 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing positive staining in mouse primary glia cells.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab194324 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).