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リコンビナントRabMAb

Alexa Fluor® 488 Anti-Fibrillarin 抗体 [EPR10823(B)] - Nucleolar Marker (ab184817)

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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab184817)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab184817)
  • Alexa Fluor® 488 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab184817)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Carrier Free HRP Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 488 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker
    Fibrillarin 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 488 Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • アプリケーション

    適用あり: ICC/IF, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184817の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/50 - 1/100.
Flow Cyt (Intra)
1/500.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.
特記事項
ICC/IF
1/50 - 1/100.
Flow Cyt (Intra)
1/500.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).

  • 配列類似性

    Belongs to the methyltransferase superfamily. Fibrillarin family.

  • 翻訳後修飾

    By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).

  • 細胞内局在

    Nucleus, nucleolus. Fibrillar region of the nucleolus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 34 kD nucleolar scleroderma antigen antibody
    • 34 kDa nucleolar scleroderma antigen antibody
    • Fbl antibody
    • FBRL_HUMAN antibody
    • FIB antibody
    • FIB1 antibody
    • FLRN antibody
    • Histone-glutamine methyltransferase antibody
    • Nop1p antibody
    • RNA U3 small nucleolar interacting protein 1 antibody
    • RNU3IP1 antibody
    • rRNA 2' O methyltransferase fibrillarin antibody
    • rRNA 2'-O-methyltransferase fibrillarin antibody
    see all

画像

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab184817)
    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab184817)

    Overlay histogram showing HeLa cells stained with ab184817 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184817, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab184817)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab184817)

    ab184817 staining Fibrillarin in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab184817 at a working diltuion of 1 in 100 overnight at +4°C (shown in green). AlexaFluor® 350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410).

  • Alexa Fluor® 488 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab184817)
    Alexa Fluor® 488 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab184817)

参考文献 (1)

ab184817 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184817 は 1 報の論文で使用されています。

  • Izzo A  et al. Nucleophosmin 1 cooperates with the methyltransferase DOT1L to preserve peri-nucleolar heterochromatin organization by regulating H3K27me3 levels and DNA repeats expression. Epigenetics Chromatin 16:36 (2023). PubMed: 37759327

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