AB204266

Alexa Fluor® 488 Anti-F4/80 抗体 [F4/80]

Alexa Fluor® 488 Anti-F4/80 antibody [F4/80]

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(5 Publications)

Rat Monoclonal F4/80 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Mouse samples. Cited in 5 publications.

別名を表示する

Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] (AB204266)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] (AB204266)

ab204266 staining F4/80 in Raw264.7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204266 at a 1/40 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

F4/80

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

標識

Alexa Fluor® 488

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

交差種

Mouse

アプリケーション

ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in Raw264.7 cells fixed with 100% methanol (5 min).</p>" } } }

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製品の詳細

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出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

See full target information Adgre1

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

BMC musculoskeletal disorders 22:494 PubMed34049522

2021

Changes in macrophage and inflammatory cytokine expressions during fracture healing in an ovariectomized mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Chen,Shao Cheng,Kanghui Sun,Jing Wang,Xinhua Liu,Yongjian Zhao,Junjie Yang,Dongfeng Zhao,Chunchun Xue,Yuren Tao,Shitian Zhao,Hao Zhang,Sheng Lu,Qi Shi,Yongjun Wang,Bing Shu

IUBMB life 72:198-213 PubMed31444954

2019

Downregulation of microRNA-135b promotes atherosclerotic plaque stabilization in atherosclerotic mice by upregulating erythropoietin receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Wen Wu,Yu Liu,Mi-Shan Wu,Yun-Hui Meng,Meng Lu,Jin-Dong Guo,Yu-Hui Zhou

PloS one 14:e0212880 PubMed30811469

2019

Deletion of suppressor of cytokine signaling 3 (SOCS3) in muscle stem cells does not alter muscle regeneration in mice after injury.

Applications

Unspecified application

Species

Unspecified reactive species

Kristy Swiderski,Marissa K Caldow,Timur Naim,Jennifer Trieu,Annabel Chee,René Koopman,Gordon S Lynch

Molecular imaging 16:1536012116689499 PubMed28654380

2017

Targeted Imaging of Tumor-Associated Macrophages by Cyanine 7-Labeled Mannose in Xenograft Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Jiang,Huawei Cai,Xiaodong Peng,Ping Zhang,Xiaoai Wu,Rong Tian

Mediators of inflammation 2017:6541729 PubMed28539706

2017

Multiple Immunosuppressive Effects of CpG-c41 on Intracellular TLR-Mediated Inflammation.

Applications

ICC/IF

Species

Mouse

Wancheng Liu,Xuejiao Yang,Ning Wang,Shijun Fan,Yuanfeng Zhu,Xinchuan Zheng,Yan Li

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

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Alexa Fluor® 488 Anti-F4/80 抗体 [F4/80]

Alexa Fluor® 488 Anti-F4/80 antibody [F4/80]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] (AB204266)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

Abcam product promise

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